View source for 3kep


==Crystal structure of the autoproteolytic domain from the nuclear pore complex component NUP145 from Saccharomyces cerevisiae==
<StructureSection load='3kep' size='340' side='right'caption='[[3kep]], [[Resolution|resolution]] 1.82&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3kep]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KEP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3KEP FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.82&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3kep FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kep OCA], [https://pdbe.org/3kep PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3kep RCSB], [https://www.ebi.ac.uk/pdbsum/3kep PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3kep ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/NU145_YEAST NU145_YEAST] Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. Active directional transport is assured by both, a Phe-Gly (FG) repeat affinity gradient for these transport factors across the NPC and a transport cofactor concentration gradient across the nuclear envelope (GSP1 and GSP2 GTPases associated predominantly with GTP in the nucleus, with GDP in the cytoplasm). NUP145 is autocatalytically cleaved in vivo in 2 polypeptides which assume different functions in the NPC. NUP145N as one of the FG repeat nucleoporins participates in karyopherin interactions and contains part of the autocatalytic cleavage activity. NUP145C as part of the NUP84 complex is involved in nuclear poly(A)+ RNA and tRNA export. It is also required for normal NPC distribution (probably through interactions with MLP1 and MLP2) and NPC assembly, as well as for normal nuclear envelope organization.<ref>PMID:8044840</ref> <ref>PMID:8195299</ref> <ref>PMID:8524308</ref> <ref>PMID:9305650</ref> <ref>PMID:10542288</ref> <ref>PMID:10638763</ref> <ref>PMID:11823431</ref> <ref>PMID:12604785</ref> <ref>PMID:15039779</ref> 
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ke/3kep_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3kep ConSurf].
<div style="clear:both"></div>

==See Also==
*[[Nucleoporin 3D structures|Nucleoporin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Atwell S]]
[[Category: Bain K]]
[[Category: Burley SK]]
[[Category: Dickey M]]
[[Category: Do J]]
[[Category: Emtage JS]]
[[Category: Fernandez-Martinez J]]
[[Category: Franke JD]]
[[Category: Gheyi T]]
[[Category: Kim SJ]]
[[Category: Ozyurt SA]]
[[Category: Phillips J]]
[[Category: Pieper U]]
[[Category: Rout M]]
[[Category: Sali A]]
[[Category: Sampathkumar P]]
[[Category: Sauder JM]]
[[Category: Thompson DA]]
[[Category: Wasserman S]]