View source for 3mbs


==Crystal structure of 8mer PNA==
<StructureSection load='3mbs' size='340' side='right'caption='[[3mbs]], [[Resolution|resolution]] 1.27&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3mbs]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MBS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3MBS FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.27&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=APN:2-AMINOETHYLGLYCINE-CARBONYLMETHYLENE-ADENINE'>APN</scene>, <scene name='pdbligand=CPN:2-AMINOETHYLGLYCINE-CARBONYLMETHYLENE-CYTOSINE'>CPN</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GPN:2-AMINOETHYLGLYCINE-CARBONYLMETHYLENE-GUANINE'>GPN</scene>, <scene name='pdbligand=TPN:2-AMINOETHYLGLYCINE-CARBONYLMETHYLENE-THYMINE'>TPN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3mbs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mbs OCA], [https://pdbe.org/3mbs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3mbs RCSB], [https://www.ebi.ac.uk/pdbsum/3mbs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3mbs ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Peptide nucleic acid (PNA) is a synthetic analogue of DNA that commonly has an N-aminoethyl glycine backbone. The crystal structures of two PNA duplexes, one containing eight standard nucleobase pairs (GGCATGCC)(2), and the other containing the same nucleobase pairs and a central pair of bipyridine ligands, have been solved with a resolution of 1.22 and 1.10 A, respectively. The non-modified PNA duplex adopts a P-type helical structure similar to that of previously characterized PNAs. The atomic-level resolution of the structures allowed us to observe for the first time specific modes of interaction between the terminal lysines of the PNA and the backbone and the nucleobases situated in the vicinity of the lysines, which are considered an important factor in the induction of a preferred handedness in PNA duplexes. Our results support the notion that whereas PNA typically adopts a P-type helical structure, its flexibility is relatively high. For example, the base-pair rise in the bipyridine-containing PNA is the largest measured to date in a PNA homoduplex. The two bipyridines bulge out of the duplex and are aligned parallel to the major groove of the PNA. In addition, two bipyridines from adjacent PNA duplexes form a pi-stacked pair that relates the duplexes within the crystal. The bulging out of the bipyridines causes bending of the PNA duplex, which is in contrast to the structure previously reported for biphenyl-modified DNA duplexes in solution, where the biphenyls are pi stacked with adjacent nucleobase pairs and adopt an intrahelical geometry. This difference shows that relatively small perturbations can significantly impact the relative position of nucleobase analogues in nucleic acid duplexes.

The crystal structure of non-modified and bipyridine-modified PNA duplexes.,Yeh JI, Pohl E, Truan D, He W, Sheldrick GM, Du S, Achim C Chemistry. 2010 Oct 18;16(39):11867-75. PMID:20859960<ref>PMID:20859960</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3mbs" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Achim C]]
[[Category: He W]]
[[Category: Pohl E]]
[[Category: Sheldrick GM]]
[[Category: Truan D]]
[[Category: Yeh JI]]