View source for 3mc2


==Crystal Structure of the Murine Inhibitor of Carbonic Anhydrase==
<StructureSection load='3mc2' size='340' side='right'caption='[[3mc2]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3mc2]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MC2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3MC2 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3mc2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mc2 OCA], [https://pdbe.org/3mc2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3mc2 RCSB], [https://www.ebi.ac.uk/pdbsum/3mc2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3mc2 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ICA_MOUSE ICA_MOUSE] Inhibitor for carbonic anhydrase 2 (CA2). Does not bind iron ions.<ref>PMID:17511619</ref> <ref>PMID:18712936</ref> 
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mc/3mc2_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3mc2 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The original signature of the transferrin (TF) family of proteins was the ability to bind ferric iron with high affinity in the cleft of each of two homologous lobes. However, in recent years new family members which do not bind iron have been discovered. One new member is the inhibitor of carbonic anhydrase (ICA), which as its name indicates, binds to and strongly inhibits certain isoforms of carbonic anhydrase. Recently mouse ICA has been expressed as a recombinant protein in a mammalian cell system. Here we describe the 2.4 A structure of mouse ICA from a pseudomerohedral twinned crystal. As predicted, the structure is bilobal, comprised of two alpha-beta domains per lobe typical of the other family members; as with all but insect TFs, the structure includes the unusual reverse gamma-turn in each lobe. The structure is consistent with the fact that introduction of two mutations in the N-lobe of mICA (W124R and S188Y) allowed it to bind iron with high affinity. Unexpectedly, both lobes of the mICA were found in the closed conformation usually associated with presence of iron in the cleft, and making the structure most similar to diferric pig TF. Two new ICA family members (guinea pig and horse) were identified from genomic sequences and used in evolutionary comparisons. Additionally, a comparison of selection pressure (dN/dS) on functional residues reveals some interesting insights into the evolution of the TF family including that the N-lobe of lactoferrin may be in the process of eliminating its iron binding function.

The structure and evolution of the murine inhibitor of carbonic anhydrase: A member of the transferrin superfamily.,Eckenroth BE, Mason AB, McDevitt ME, Lambert LA, Everse SJ Protein Sci. 2010 Jun 23. PMID:20572014<ref>PMID:20572014</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3mc2" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Eckenroth BE]]
[[Category: Everse SJ]]
[[Category: Mason AB]]