View source for 3muy


==E. coli (lacZ) beta-galactosidase (R599A)==
<StructureSection load='3muy' size='340' side='right'caption='[[3muy]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3muy]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MUY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3MUY FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3muy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3muy OCA], [https://pdbe.org/3muy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3muy RCSB], [https://www.ebi.ac.uk/pdbsum/3muy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3muy ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/BGAL_ECOLI BGAL_ECOLI] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Structural and kinetic data show that Arg-599 of beta-galactosidase plays an important role in anchoring the "open" conformations of both Phe-601 and an active-site loop (residues 794-803). When alanine was substituted for Arg-599, the conformations of Phe-601 and the loop shifted towards the "closed" positions because interactions with the guanidinium side chain were lost. Also, Phe-601, the loop, and Na+, which is ligated by the backbone carbonyl of Phe-601, lost structural order, as indicated by large B-factors. IPTG, a substrate analog, restored the conformations of Phe-601 and the loop of R599A-beta-galactosidase to the open state found with IPTG-complexed native enzyme and partially reinstated order. -Galactonolactone, a transition state analog, restored the closed conformations of R599A-beta-galactosidase to those found with -galactonolactone-complexed native enzyme and completely re-established the order. Substrates and substrate analogs bound R599A-beta-galactosidase with less affinity because the closed conformation does not allow substrate binding and extra energy is required for Phe-601 and the loop to open. In contrast, transition state analog binding, which occurs best when the loop is closed, was several-fold better. The higher energy level of the enzyme*substrate complex and the lower energy level of the first transition state means that less activation energy is needed to form the first transition state and thus the rate of the first catalytic step (k2) increased substantially. The rate of the second catalytic step (k3) decreased, likely because the covalent form is more stabilized than the second transition state when Phe-601 and the loop are closed. The importance of the guanidinium group of Arg-599 was confirmed by restoration of conformation, order, and activity by guanidinium ions.

Importance of Arg-599 of beta-galactosidase (Escherichia coli) as an anchor for the open conformations of Phe-601 and the active-site loop.,Dugdale ML, Vance ML, Wheatley RW, Driedger MR, Nibber A, Tran A, Huber RE Biochem Cell Biol. 2010 Dec;88(6):969-79. PMID:21102659<ref>PMID:21102659</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3muy" style="background-color:#fffaf0;"></div>

==See Also==
*[[Galactosidase 3D structures|Galactosidase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli K-12]]
[[Category: Large Structures]]
[[Category: Driedger ML]]
[[Category: Dugdale ML]]
[[Category: Huber RE]]
[[Category: Nibber A]]
[[Category: Tran A]]
[[Category: Vance M]]