View source for 3mvc


==High resolution crystal structure of the heme domain of GLB-6 from C. elegans==
<StructureSection load='3mvc' size='340' side='right'caption='[[3mvc]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3mvc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MVC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3MVC FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.401&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=PR:PRASEODYMIUM+ION'>PR</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3mvc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mvc OCA], [https://pdbe.org/3mvc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3mvc RCSB], [https://www.ebi.ac.uk/pdbsum/3mvc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3mvc ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/GLOB6_CAEEL GLOB6_CAEEL] May play a role as physiological sensor for oxygen via redox signaling and/or electron transport.<ref>PMID:20518498</ref> 
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mv/3mvc_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3mvc ConSurf].
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== Publication Abstract from PubMed ==
Globins are heme-containing proteins that are best known for their roles in oxygen (O2) transport and storage. However, more diverse roles of globins in biology are being revealed, including gas and redox sensing. In the nematode Caenorhabditis elegans, 33 globin or globin-like genes have been recently identified, some of which are known to be expressed in the sensory neurons of the worm and linked to O2 sensing behavior. Here, we describe GLB-6, a novel globin-like protein expressed in the neurons of C. elegans. Recombinantly expressed full-length GLB-6 contains a heme site with spectral features that are similar to those of other bis-histidyl ligated globins, such as neuroglobin and cytoglobin. In contrast to these globins, however, ligands such as CO, NO, and CN- do not bind to the heme in GLB-6, demonstrating that the endogenous histidine ligands are likely very tightly coordinated. Additionally, GLB-6 exhibits rapid two-state autoxidation kinetics in the presence of physiological O2 levels as well as a low redox potential (-193 +/- 2 mV). A high resolution (1.40 A) crystal structure of the ferric form of the heme-domain of GLB-6 confirms both the putative globin-fold and bis-histidyl ligation and also demonstrates key structural features that can be correlated with the unusual ligand binding and redox properties exhibited by the full-length protein. Taken together, the biochemical properties of GLB-6 suggest that this neural protein would most likely serve as a physiological sensor for O2 in C. elegans via redox signaling and/or electron transfer.

Structure and Properties of a Bis-Histidyl Ligated Globin from Caenorhabditis elegans.,Yoon J, Herzik MA, Winter MB, Tran R, Olea C, Marletta MA Biochemistry. 2010 Jun 2. PMID:20518498<ref>PMID:20518498</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Caenorhabditis elegans]]
[[Category: Large Structures]]
[[Category: Herzik Jr MA]]
[[Category: Marletta MA]]
[[Category: Olea Jr C]]
[[Category: Tran R]]
[[Category: Winter MB]]
[[Category: Yoon J]]