View source for 3nbc


==Clitocybe nebularis ricin B-like lectin (CNL) in complex with lactose, crystallized at pH 4.4==
<StructureSection load='3nbc' size='340' side='right'caption='[[3nbc]], [[Resolution|resolution]] 1.01&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3nbc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Clitocybe_nebularis Clitocybe nebularis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NBC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NBC FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.01&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=PRD_900004:beta-lactose'>PRD_900004</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nbc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nbc OCA], [https://pdbe.org/3nbc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nbc RCSB], [https://www.ebi.ac.uk/pdbsum/3nbc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nbc ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CNL_CLINE CNL_CLINE] Lectin specific for terminal, non-reducing N-acetylgalactosamine (Gal-NAc)-containing carbohydrates including N,N'-diacetyllactosediamine/LDN (GalNAcbeta1-4GlcNAc, LacdiNAc). Specific also for carbohydrates containing N-acetylglucosamine (-GlcNAc) or N-acetyllactosamine (-Galbeta1-4GlcNAc) at the reducing end. Agglutinates human blood group A, AB, B and O erythrocytes with a strong preference for group A. Agglutinates bovine erythrocytes with a very low specificity (PubMed:19100814, PubMed:22298779). Binds carbohydrates bivalently, which is required for its biological activity (PubMed:22298779). Exhibits insecticidal activity against the fruit fly D.melanogaster, mosquito A.aegypti, and amoebozoa A.castellanii. Has anti-nutritional activity against Colorado potato beetle L.decemlineata, and against worm C.elegans (PubMed:21556921, PubMed:21486374). Has antiproliferative activity against human leukemic T-cells (PubMed:19100814). Has an immunostimulatory effect on human antigen-presenting dendritic cells, which are subsequently able to induce efficient T-cell immune responses (PubMed:22044067).<ref>PMID:19100814</ref> <ref>PMID:21486374</ref> <ref>PMID:21556921</ref> <ref>PMID:22044067</ref> <ref>PMID:22298779</ref> 

==See Also==
*[[Ricin 3D structures|Ricin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Clitocybe nebularis]]
[[Category: Large Structures]]
[[Category: Kos J]]
[[Category: Pohleven J]]
[[Category: Renko M]]
[[Category: Sabotic J]]
[[Category: Turk D]]