View source for 3nda


==Crystal structure of serpin from tick Ixodes ricinus==
<StructureSection load='3nda' size='340' side='right'caption='[[3nda]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3nda]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ixodes_ricinus Ixodes ricinus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NDA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NDA FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nda FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nda OCA], [https://pdbe.org/3nda PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nda RCSB], [https://www.ebi.ac.uk/pdbsum/3nda PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nda ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q06B74_IXORI Q06B74_IXORI] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Platelet aggregation and acute inflammation are key processes in vertebrate defense to a skin injury. Recent studies uncovered the mediation of two serine proteases, cathepsin G and chymase, in both mechanisms. Working with a mouse model of acute inflammation, we revealed that an exogenous salivary protein of Ixodes ricinus, the vector of Lyme disease pathogens in Europe, extensively inhibits edema formation and influx of neutrophils in the inflamed tissue. We named this tick salivary gland secreted effector as Ixodes ricinus serpin-2 (IRS-2) and we show that it primarily inhibits cathepsin G and chymase, while in higher molar excess it affects thrombin activity as well. The inhibitory specificity was explained using the crystal structure, determined at a resolution of 1.8 A. Moreover, we disclosed the ability of IRS-2 to inhibit cathepsin G-induced and thrombin-induced platelet aggregation. For the first time, an ectoparasite protein is shown to exhibit such pharmacological effects and target specificity. The stringent specificity and the biological activities of IRS-2 combined with the knowledge of its structure can be the basis for the development of future pharmaceutical applications.

A tick salivary protein targets cathepsin G and chymase and inhibits host inflammation and platelet aggregation.,Chmelar J, Oliveira CJ, Rezacova P, Francischetti IM, Kovarova Z, Pejler G, Kopacek P, Ribeiro JM, Mares M, Kopecky J, Kotsyfakis M Blood. 2010 Oct 12. PMID:20940421<ref>PMID:20940421</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3nda" style="background-color:#fffaf0;"></div>

==See Also==
*[[Serpin 3D structures|Serpin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Ixodes ricinus]]
[[Category: Large Structures]]
[[Category: Chmelar J]]
[[Category: Kovarova Z]]
[[Category: Mares M]]
[[Category: Rezacova P]]