View source for 3nfq


==Crystal structure of the conserved central domain of yeast Spn1/Iws1==
<StructureSection load='3nfq' size='340' side='right'caption='[[3nfq]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3nfq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NFQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NFQ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nfq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nfq OCA], [https://pdbe.org/3nfq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nfq RCSB], [https://www.ebi.ac.uk/pdbsum/3nfq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nfq ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/IWS1_YEAST IWS1_YEAST] Transcription factor involved in RNA polymerase II transcription regulation. May function in both SPT15/TBP post-recruitment and recruitment steps of transcription.<ref>PMID:12524336</ref> <ref>PMID:12556496</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Spn1 plays essential roles in the regulation of gene expression by RNA polymerase II (RNAPII), and it is highly conserved in organisms ranging from yeast to humans. Spn1 physically and/or genetically interacts with RNAPII, TBP (TATA-binding protein), TFIIS (transcription factor IIS), and a number of chromatin remodeling factors (Swi-Snf and Spt6). The central domain of Spn1 (residues 141-305 out of 410) is necessary and sufficient for performing the essential functions of SPN1 in yeast cells. Here, we report the high-resolution (1.85 A) crystal structure of the conserved central domain of Saccharomyces cerevisiae Spn1. The central domain is composed of eight alpha-helices in a right-handed superhelical arrangement and exhibits structural similarity to domain I of TFIIS. A unique structural feature of Spn1 is a highly conserved loop, which defines one side of a pronounced cavity. The loop and the other residues forming the cavity are highly conserved at the amino acid level among all Spn1 family members, suggesting that this is a signature motif for Spn1 orthologs. The locations and the molecular characterization of temperature-sensitive mutations in Spn1 indicate that the cavity is a key attribute of Spn1 that is critical for its regulatory functions during RNAPII-mediated transcriptional activity.

The Transcription Factor Spn1 Regulates Gene Expression via a Highly Conserved Novel Structural Motif.,Pujari V, Radebaugh CA, Chodaparambil JV, Muthurajan UM, Almeida AR, Fischbeck JA, Luger K, Stargell LA J Mol Biol. 2010 Sep 25. PMID:20875428<ref>PMID:20875428</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3nfq" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Luger K]]
[[Category: Stargell LA]]