View source for 3nzm


==Crystal structure of DNAE intein with N-extein in redox trap==
<StructureSection load='3nzm' size='340' side='right'caption='[[3nzm]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3nzm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechocystis_sp._PCC_6803 Synechocystis sp. PCC 6803]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NZM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NZM FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SNN:L-3-AMINOSUCCINIMIDE'>SNN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nzm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nzm OCA], [https://pdbe.org/3nzm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nzm RCSB], [https://www.ebi.ac.uk/pdbsum/3nzm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nzm ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/DPO3A_SYNY3 DPO3A_SYNY3] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Here we describe self-splicing proteins, called inteins, that function as redox-responsive switches in bacteria. Redox regulation was achieved by engineering a disulfide bond between the intein's catalytic cysteine and a cysteine in the flanking 'extein' sequence. This interaction was validated by an X-ray structure, which includes a transient splice junction. A natural analog of the designed system was identified in Pyrococcus abyssi, suggesting an unprecedented form of adaptive, post-translational regulation.

Structure of catalytically competent intein caught in a redox trap with functional and evolutionary implications.,Callahan BP, Topilina NI, Stanger MJ, Van Roey P, Belfort M Nat Struct Mol Biol. 2011 May;18(5):630-3. Epub 2011 Apr 3. PMID:21460844<ref>PMID:21460844</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3nzm" style="background-color:#fffaf0;"></div>

==See Also==
*[[DNA polymerase 3D structures|DNA polymerase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Synechocystis sp. PCC 6803]]
[[Category: Belfort M]]
[[Category: Callahan BP]]
[[Category: Van Roey P]]