View source for 3ot9


==Phosphopentomutase from Bacillus cereus bound to glucose-1,6-bisphosphate==
<StructureSection load='3ot9' size='340' side='right'caption='[[3ot9]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3ot9]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_cereus_ATCC_14579 Bacillus cereus ATCC 14579]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OT9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OT9 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G16:ALPHA-D-GLUCOSE+1,6-BISPHOSPHATE'>G16</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ot9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ot9 OCA], [https://pdbe.org/3ot9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ot9 RCSB], [https://www.ebi.ac.uk/pdbsum/3ot9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ot9 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/DEOB_BACCR DEOB_BACCR] Phosphotransfer between the C1 and C5 carbon atoms of pentose (By similarity).
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== Publication Abstract from PubMed ==
Bacterial phosphopentomutases (PPMs) are alkaline phosphatase superfamily members that interconvert alpha-D-ribose-5-phosphate (ribose-5-phosphate) and alpha-D-ribose-1-phosphate (ribose-1-phosphate). We investigated the reaction mechanism of Bacillus cereus PPM using a combination of structural and biochemical studies. Four high-resolution crystal structures of B. cereus PPM revealed the active site architecture, identified binding sites for the substrate ribose-5-phosphate and the activator alpha-D-glucose-1,6-bisphosphate (glucose-1,6-bisphosphate), and demonstrated that glucose-1,6-bisphosphate increased phosphorylation of the active site residue Thr-85. The phosphorylation of Thr-85 was confirmed by Western and mass spectroscopic analyses. Biochemical assays identified Mn2+-dependent enzyme turnover and demonstrated that glucose-1,6-bisphosphate treatment increases enzyme activity. These results suggest that protein phosphorylation activates the enzyme, which supports an intermolecular transferase mechanism. We confirmed intermolecular phosphoryl transfer using an isotope relay assay in which PPM reactions containing mixtures of ribose-5-[18O3]-phosphate and 13C5-ribose-5-phosphate were analyzed by mass spectrometry. This intermolecular phosphoryl transfer is seemingly counter to what is anticipated from phosphomutases employing a general alkaline phosphatase reaction mechanism, which are reported to catalyze intramolecular phosphoryl transfer. However, the mechanism may be reconciled with intermolecular transfer if substrate encounters the enzyme at a different point in the catalytic cycle.

The Bacillus cereus phosphopentomutase is an alkaline phosphatase family member that exhibits an altered entry point into the catalytic cycle.,Panosian TD, Nannemann DP, Watkins G, Phelan VV, McDonald WH, Wadzinski BE, Bachmann BO, Iverson TM J Biol Chem. 2010 Dec 30. PMID:21193409<ref>PMID:21193409</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 3ot9" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bacillus cereus ATCC 14579]]
[[Category: Large Structures]]
[[Category: Bachmann BO]]
[[Category: Iverson TM]]
[[Category: Nannemann DP]]
[[Category: Panosian TD]]
[[Category: Phalen V]]
[[Category: Wadzinski B]]
[[Category: Watkins G]]