View source for 3p9u


==Crystal structure of TetX2 from Bacteroides thetaiotaomicron with substrate analogue==
<StructureSection load='3p9u' size='340' side='right'caption='[[3p9u]], [[Resolution|resolution]] 2.81&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3p9u]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacteroides_thetaiotaomicron Bacteroides thetaiotaomicron]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P9U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3P9U FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.81&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3p9u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p9u OCA], [https://pdbe.org/3p9u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3p9u RCSB], [https://www.ebi.ac.uk/pdbsum/3p9u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3p9u ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
We have determined the structure of Bacteroides thetaiotaomicron TetX2 at 2.8 A resolution, and shown that it is a class A flavin dependent oxidoreductase. TetX2 has broad activity against a range of tetracyclines including one of the most recent tetracyclines, tigecycline (Tygacil((R))). Comparison of TetX2 with that of the weakly homologous Pseudomonas fluorescens para-hydroxybenzoate hydroxylase (PHBH) (21% identity) shows substantial differences among residues at the substrate binding site although FAD is positioned in a similar conformation between the two enzymes and is poised to carry out catalysis.

Crystal structure of Bacteroides thetaiotaomicron TetX2: a tetracycline degrading monooxygenase at 2.8 A resolution.,Walkiewicz K, Davlieva M, Wu G, Shamoo Y Proteins. 2011 Jul;79(7):2335-40. doi: 10.1002/prot.23052. Epub 2011 May 16. PMID:21590745<ref>PMID:21590745</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 3p9u" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bacteroides thetaiotaomicron]]
[[Category: Large Structures]]
[[Category: Davlieva M]]
[[Category: Lau K]]
[[Category: Shamoo Y]]
[[Category: Sun C]]
[[Category: Walkiewicz K]]