View source for 3pla


==Crystal structure of a catalytically active substrate-bound box C/D RNP from Sulfolobus solfataricus==
<StructureSection load='3pla' size='340' side='right'caption='[[3pla]], [[Resolution|resolution]] 3.15&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3pla]] is a 15 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus Saccharolobus solfataricus], [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus_98/2 Saccharolobus solfataricus 98/2] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PLA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PLA FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.15&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pla FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pla OCA], [https://pdbe.org/3pla PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pla RCSB], [https://www.ebi.ac.uk/pdbsum/3pla PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pla ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/D0KRE2_SACS9 D0KRE2_SACS9] Multifunctional RNA-binding protein that recognizes the K-turn motif in ribosomal RNA, the RNA component of RNase P, box H/ACA, box C/D and box C'/D' sRNAs.[HAMAP-Rule:MF_00326]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Box C/D RNA protein complexes (RNPs) direct site-specific 2'-O-methylation of RNA and ribosome assembly. The guide RNA in C/D RNP forms base pairs with complementary substrates and selects the modification site using a molecular ruler. Despite many studies of C/D RNP structure, the fundamental questions of how C/D RNAs assemble into RNPs and how they guide modification remain unresolved. Here we report the crystal structure of an entire catalytically active archaeal C/D RNP consisting of a bipartite C/D RNA associated with two substrates and two copies each of Nop5, L7Ae and fibrillarin at 3.15-A resolution. The substrate pairs with the second through the eleventh nucleotide of the 12-nucleotide guide, and the resultant duplex is bracketed in a channel with flexible ends. The methyltransferase fibrillarin binds to an undistorted A-form structure of the guide-substrate duplex and specifically loads the target ribose into the active site. Because interaction with the RNA duplex alone does not determine the site specificity, fibrillarin is further positioned by non-specific and specific protein interactions. Compared with the structure of the inactive C/D RNP, extensive domain movements are induced by substrate loading. Our results reveal the organization of a monomeric C/D RNP and the mechanism underlying its site-specific methylation activity.

Structural basis for site-specific ribose methylation by box C/D RNA protein complexes.,Lin J, Lai S, Jia R, Xu A, Zhang L, Lu J, Ye K Nature. 2011 Jan 27;469(7331):559-563. PMID:21270896<ref>PMID:21270896</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3pla" style="background-color:#fffaf0;"></div>

==See Also==
*[[Kink-turn motif|Kink-turn motif]]
*[[Ribosomal protein L7/L12|Ribosomal protein L7/L12]]
*[[User:Wayne Decatur/kink-turn motif|User:Wayne Decatur/kink-turn motif]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharolobus solfataricus]]
[[Category: Saccharolobus solfataricus 98/2]]
[[Category: Synthetic construct]]
[[Category: Jia R]]
[[Category: Lai S]]
[[Category: Lin J]]
[[Category: Lu J]]
[[Category: Xu A]]
[[Category: Ye K]]
[[Category: Zhang L]]