View source for 3pq1


==Crystal structure of human mitochondrial poly(A) polymerase (PAPD1)==
<StructureSection load='3pq1' size='340' side='right'caption='[[3pq1]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3pq1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PQ1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PQ1 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pq1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pq1 OCA], [https://pdbe.org/3pq1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pq1 RCSB], [https://www.ebi.ac.uk/pdbsum/3pq1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pq1 ProSAT]</span></td></tr>
</table>
== Disease ==
[https://www.uniprot.org/uniprot/PAPD1_HUMAN PAPD1_HUMAN] Autosomal recessive spastic ataxia - optic atrophy - dysarthria. The disease is caused by mutations affecting the gene represented in this entry. MTPAP mutations result in a defect of mitochondrial mRNA maturation. Affected individuals exhibit a drastic decrease in poly(A) tail length of mitochondrial mRNA transcripts, including COX1 and RNA14 (PubMed:20970105).<ref>PMID:20970105</ref> 
== Function ==
[https://www.uniprot.org/uniprot/PAPD1_HUMAN PAPD1_HUMAN] Polymerase that creates the 3' poly(A) tail of mitochondrial transcripts. Can use all four nucleotides, but has higher activity with ATP and UTP (in vitro). Plays a role in replication-dependent histone mRNA degradation. May be involved in the terminal uridylation of mature histone mRNAs before their degradation is initiated. Might be responsible for the creation of some UAA stop codons which are not encoded in mtDNA.<ref>PMID:15547249</ref> <ref>PMID:15769737</ref> <ref>PMID:18172165</ref> <ref>PMID:20970105</ref> <ref>PMID:21292163</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Poly(A) polymerases (PAPs) are found in most living organisms and have important roles in RNA function and metabolism. Here, we report the crystal structure of human PAPD1, a noncanonical PAP that can polyadenylate RNAs in the mitochondria (also known as mtPAP) and oligouridylate histone mRNAs (TUTase1). The overall structure of the palm and fingers domains is similar to that in the canonical PAPs. The active site is located at the interface between the two domains, with a large pocket that can accommodate the substrates. The structure reveals the presence of a previously unrecognized domain in the N-terminal region of PAPD1, with a backbone fold that is similar to that of RNP-type RNA binding domains. This domain (named the RL domain), together with a beta-arm insertion in the palm domain, contributes to dimerization of PAPD1. Surprisingly, our mutagenesis and biochemical studies show that dimerization is required for the catalytic activity of PAPD1.

Structural basis for dimerization and activity of human PAPD1, a noncanonical poly(A) polymerase.,Bai Y, Srivastava SK, Chang JH, Manley JL, Tong L Mol Cell. 2011 Feb 4;41(3):311-20. PMID:21292163<ref>PMID:21292163</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3pq1" style="background-color:#fffaf0;"></div>

==See Also==
*[[Poly(A) RNA polymerase|Poly(A) RNA polymerase]]
*[[Poly(A) polymerase 3D structures|Poly(A) polymerase 3D structures]]
*[[RNA uridylyltransferase|RNA uridylyltransferase]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Bai Y]]
[[Category: Chang JH]]
[[Category: Srivastava SK]]
[[Category: Tong L]]