View source for 3pqk


==Crystal Structure of the transcriptional repressor BigR from Xylella fastidiosa==
<StructureSection load='3pqk' size='340' side='right'caption='[[3pqk]], [[Resolution|resolution]] 2.09&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3pqk]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Xylella_fastidiosa Xylella fastidiosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PQK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PQK FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.09&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pqk OCA], [https://pdbe.org/3pqk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pqk RCSB], [https://www.ebi.ac.uk/pdbsum/3pqk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pqk ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/BIGR_XYLFA BIGR_XYLFA] Represses an operon that comprises itself, XF_0764, XF_0765, XF_0766 and blh. Binds to a palindromic AT-rich sequence spanning the -10 region of the blh promoter and blocks transcription of the operon.<ref>PMID:17586627</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Winged-helix transcriptional factors play important roles in the control of gene expression in many organisms. In the plant pathogens Xylella fastidiosa and Agrobacterium tumefaciens, the winged-helix protein BigR, a member of the ArsR/SmtB family of metal sensors, regulates transcription of the bigR operon involved in bacterial biofilm growth. Previous studies showed that BigR represses transcription of its own operon through the occupation of the RNA polymerase-binding site; however, the signals that modulate its activity and the biological function of its operon are still poorly understood. Here we show that although BigR is a homodimer similar to metal sensors, it functions as a novel redox switch that derepresses transcription upon oxidation. Crystal structures of reduced and oxidized BigR reveal that formation of a disulfide bridge involving two critical cysteines induces conformational changes in the dimer that remarkably alter the topography of the winged-helix DNA-binding interface, precluding DNA binding. This structural mechanism of DNA association-dissociation is novel among winged-helix factors. Moreover, we demonstrate that the bigR operon is required for hydrogen sulfide detoxification through the action of a sulfur dioxygenase (Blh) and sulfite exporter. As hydrogen sulfide strongly inhibits cytochrome c oxidase, it must be eliminated to allow aerobic growth under low oxygen tension, an environmental condition found in bacterial biofilms, xylem vessels, and root tissues. Accordingly, we show that the bigR operon is critical to sustain bacterial growth under hypoxia. These results suggest that BigR integrates the transcriptional regulation of a sulfur oxidation pathway to an oxidative signal through a thiol-based redox switch.

Plant Pathogenic Bacteria Utilize Biofilm Growth-associated Repressor (BigR), a Novel Winged-helix Redox Switch, to Control Hydrogen Sulfide Detoxification under Hypoxia.,Guimaraes BG, Barbosa RL, Soprano AS, Campos BM, de Souza TA, Tonoli CC, Leme AF, Murakami MT, Benedetti CE J Biol Chem. 2011 Jul 22;286(29):26148-57. Epub 2011 Jun 1. PMID:21632538<ref>PMID:21632538</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3pqk" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Xylella fastidiosa]]
[[Category: Benedetti CE]]
[[Category: Guimaraes BG]]