View source for 3pto


==Crystal Structure of an empty Vesicular Stomatitis Virus Nucleocapsid Protein Complex==
<StructureSection load='3pto' size='340' side='right'caption='[[3pto]], [[Resolution|resolution]] 3.01&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3pto]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Vesicular_stomatitis_Indiana_virus Vesicular stomatitis Indiana virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PTO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PTO FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.008&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IUM:URANYL+(VI)+ION'>IUM</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pto FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pto OCA], [https://pdbe.org/3pto PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pto RCSB], [https://www.ebi.ac.uk/pdbsum/3pto PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pto ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/NCAP_VSIVA NCAP_VSIVA] Encapsidates the genome in a ratio of one N per nine ribonucleotides, protecting it from nucleases. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. Replication is dependent on intracellular concentration of newly synthesized N, termed N(0), which corresponds to the protein not associated with RNA. In contrast, when associated with RNA, it is termed N. During replication, encapsidation by N(0) is coupled to RNA synthesis and all replicative products are resistant to nucleases (By similarity).
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== Publication Abstract from PubMed ==
The genomic RNA of negative strand RNA viruses, such as vesicular stomatitis virus (VSV), is completely enwrapped by the nucleocapsid protein (N) in every stage of virus infection. During viral transcription/replication, however, the genomic RNA in the nucleocapsid must be accessible by the virus-encoded RNA-dependent RNA polymerase in order to serve as the template for RNA synthesis. With the VSV nucleocapsid and a nucleocapsid-like particle (NLP) produced in E. coli, we have found that the RNA in the VSV nucleocapsid can be removed by RNase A, in contrast to what was previously reported. Removal of the RNA did not disrupt the assembly of the N protein, resulting in an empty capsid. Poly-ribonucleotides could be re-encapsidated into the empty NLP and the crystal structures were determined. The crystal structures revealed variable degrees of association of the N protein with a specific RNA sequence.

Access of RNA encapsidated in the nucleocapsid of vesicular stomatitis virus.,Green TJ, Rowse M, Tsao J, Kang J, Ge P, Zhou ZH, Luo M J Virol. 2010 Dec 22. PMID:21177817<ref>PMID:21177817</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3pto" style="background-color:#fffaf0;"></div>

==See Also==
*[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]]
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Vesicular stomatitis Indiana virus]]
[[Category: Green TJ]]
[[Category: Luo M]]
[[Category: Rowse M]]