View source for 3pve


==Crystal structure of the G2 domain of Agrin from Mus Musculus==
<StructureSection load='3pve' size='340' side='right'caption='[[3pve]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3pve]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PVE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PVE FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pve FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pve OCA], [https://pdbe.org/3pve PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pve RCSB], [https://www.ebi.ac.uk/pdbsum/3pve PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pve ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/AGRIN_MOUSE AGRIN_MOUSE] Heparan sulfate basal lamina glycoprotein that plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ) and directs key events in postsynaptic differentiation. This neuron-specific (z+) isoform is a component of the AGRN-LRP4 receptor complex that induces the phosphorylation and activation of MUSK. The activation of MUSK in myotubes induces the formation of NMJ by regulating different processes including the transcription of specific genes and the clustering of AChR in the postsynaptic membrane. Calcium ions are required for maximal AChR clustering. AGRN function in neurons is highly regulated by alternative splicing, glycan binding and proteolytic processing. Modulates calcium ion homeostasis in neurons, specifically by inducing an increase in cytoplasmic calcium ions. Functions differentially in the central nervous system (CNS) by inhibiting the alpha(3)-subtype of Na+/K+-ATPase and evoking depolarization at CNS synapses. This transmembrane agrin (TM-agrin) isoform, the predominate form in neurons of the brain, induces dendritic filopodia and synapse formation in mature hippocampal neurons in large part due to the attached glycosaminoglycan chains and the action of Rho-family GTPases.  Isoform 2 and isoform 3: these isoforms lacking the 'z' insert (z0) are muscle-specific, have no AChR clustering ability and may be involved in nervous system endothelial cell differentiation.  Involved in regulation of neurite outgrowth probably due to the presence of the glycosaminoglcan (GAG) side chains of heparan and chondroitin sulfate attached to the Ser/Thr- and Gly/Ser-rich regions. Also involved in modulation of growth factor signaling (By similarity).<ref>PMID:10402191</ref> <ref>PMID:11018052</ref> <ref>PMID:12796478</ref> <ref>PMID:17611272</ref> <ref>PMID:19303856</ref> <ref>PMID:21885656</ref> <ref>PMID:8653787</ref>   This released fragment is important for agrin signaling and to exert a maximal dendritic filopodia-inducing effect. All 'z' splice variants (z+) of this fragment also show an increase in the number of filopodia.

==See Also==
*[[Agrin 3D structures|Agrin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Atwell S]]
[[Category: Bain K]]
[[Category: Burley SK]]
[[Category: Do J]]
[[Category: Emtage JS]]
[[Category: Freeman J]]
[[Category: Gheyi T]]
[[Category: Sampathkumar P]]
[[Category: Sauder JM]]
[[Category: Thompson DA]]
[[Category: Wasserman S]]