View source for 3qpz


==Crystal structure of the N59A mutant of the 3-deoxy-d-manno-octulosonate 8-phosphate synthase (KDO8PS) from Neisseria meningitidis==
<StructureSection load='3qpz' size='340' side='right'caption='[[3qpz]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3qpz]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Neisseria_meningitidis_MC58 Neisseria meningitidis MC58]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QPZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QPZ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qpz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qpz OCA], [https://pdbe.org/3qpz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qpz RCSB], [https://www.ebi.ac.uk/pdbsum/3qpz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qpz ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/KDSA_NEIMB KDSA_NEIMB] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
3-Deoxy-D-&lt;i&gt;manno&lt;/i&gt;-octulosonate 8-phosphate synthase (KDO8PS) catalyzes the reaction between three-carbon phosphoenolpyruvate (PEP) and five-carbon D-arabinose 5-phosphate (A5P), generating KDO8P, a key intermediate in the biosynthetic pathway to 3-deoxy-D-&lt;i&gt;manno&lt;/i&gt;-octulosonate, a component of the lipopolysaccharide of the Gram-negative bacterial cell wall. Both metal-dependent and metal-independent forms of KDO8PS have been characterized. KDO8PS is evolutionarily and mechanistically related to the first enzyme of the shikimate pathway, the obligately divalent metal-ion-dependent 3-deoxy-D-&lt;i&gt;arabino&lt;/i&gt;-heptulosonate 7-phosphate synthase (DAH7PS) that couples PEP and four-carbon D-erythrose 4-phosphate (E4P) to give DAH7P. In KDO8PS an absolutely conserved KANRS motif forms part of the A5P binding site, whereas in DAH7PS an absolutely conserved KPR(S/T) motif accommodates E4P. Here, we have characterized four mutants of this motif (AANRS, KAARS, KARS and KPRS) in metal-dependent KDO8PS from &lt;i&gt;Acidithiobacillus ferrooxidans&lt;/i&gt; and metal-independent KDO8PS from &lt;i&gt;Neisseria meningitidis&lt;/i&gt; in order to test the roles of the universal Lys and the AlaAsn portion of the KANRS motif. The X-ray structures, determined for the &lt;i&gt;N. meningitidis&lt;/i&gt; KDO8PS mutants, indicated no gross structural penalty resulting from mutation, but the subtle changes observed in the active sites of these mutant proteins correlated with their altered catalytic function: 1) the AANRS mutations destroyed catalytic activity; 2) the KAARS mutations lowered substrate selectivity, as well as activity; 3) replacing KANRS by KARS or KPRS destroyed KDO8PS activity, but did not produce a functional DAH7PS. Thus, Lys is critical to catalysis and other changes are necessary to switch substrate specificity for both the metal-independent and metal-dependent forms of these enzymes.&lt;i&gt;&lt;/i&gt;

Targeting the role of a key conserved motif for substrate selection and catalysis by 3-deoxy-D-&lt;i&gt;manno&lt;/i&gt;-octulosonate 8-phosphate synthase.,Allison TM, Hutton RD, Cochrane FC, Yeoman JA, Jameson GB, Parker EJ Biochemistry. 2011 Mar 25. PMID:21438567<ref>PMID:21438567</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3qpz" style="background-color:#fffaf0;"></div>

==See Also==
*[[Kdo-8-phosphate synthase|Kdo-8-phosphate synthase]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Neisseria meningitidis MC58]]
[[Category: Allison TM]]
[[Category: Cochrane FC]]
[[Category: Jameson GB]]
[[Category: Parker EJ]]