View source for 3rbh


==Structure of alginate export protein AlgE from Pseudomonas aeruginosa==
<StructureSection load='3rbh' size='340' side='right'caption='[[3rbh]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3rbh]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RBH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RBH FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.301&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C8E:(HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE'>C8E</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rbh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rbh OCA], [https://pdbe.org/3rbh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rbh RCSB], [https://www.ebi.ac.uk/pdbsum/3rbh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rbh ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ALGE_PSEAE ALGE_PSEAE] Has non-porin-like, channel-forming properties and probably functions as an alginate permeability pore.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Pseudomonas aeruginosa is the predominant pathogen associated with chronic lung infection among cystic fibrosis patients. During colonization of the lung, P. aeruginosa converts to a mucoid phenotype characterized by the overproduction of the exopolysaccharide alginate. Secretion of newly synthesized alginate across the outer membrane is believed to occur through the outer membrane protein AlgE. Here we report the 2.3 A crystal structure of AlgE, which reveals a monomeric 18-stranded beta-barrel characterized by a highly electropositive pore constriction formed by an arginine-rich conduit that likely acts as a selectivity filter for the negatively charged alginate polymer. Interestingly, the pore constriction is occluded on either side by extracellular loop L2 and an unusually long periplasmic loop, T8. In halide efflux assays, deletion of loop T8 (DeltaT8-AlgE) resulted in a threefold increase in anion flux compared to the wild-type or DeltaL2-AlgE supporting the idea that AlgE forms a transport pathway through the membrane and suggesting that transport is regulated by T8. This model is further supported by in vivo experiments showing that complementation of an algE deletion mutant with DeltaT8-AlgE impairs alginate production. Taken together, these studies support a mechanism for exopolysaccharide export across the outer membrane that is distinct from the Wza-mediated translocation observed in canonical capsular polysaccharide export systems.

Structural basis for alginate secretion across the bacterial outer membrane.,Whitney JC, Hay ID, Li C, Eckford PD, Robinson H, Amaya MF, Wood LF, Ohman DE, Bear CE, Rehm BH, Lynne Howell P Proc Natl Acad Sci U S A. 2011 Aug 9;108(32):13083-8. Epub 2011 Jul 21. PMID:21778407<ref>PMID:21778407</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3rbh" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Amaya MF]]
[[Category: Bear CE]]
[[Category: Eckford PD]]
[[Category: Hay ID]]
[[Category: Howell PL]]
[[Category: Li C]]
[[Category: Ohman DE]]
[[Category: Rehm BH]]
[[Category: Robinson H]]
[[Category: Whitney JC]]
[[Category: Wood LF]]