View source for 3smz


==Human raver1 RRM1-3 domains (residues 39-320)==
<StructureSection load='3smz' size='340' side='right'caption='[[3smz]], [[Resolution|resolution]] 1.99&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3smz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SMZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SMZ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.99&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3smz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3smz OCA], [https://pdbe.org/3smz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3smz RCSB], [https://www.ebi.ac.uk/pdbsum/3smz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3smz ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RAVR1_HUMAN RAVR1_HUMAN] Cooperates with PTBP1 to modulate regulated alternative splicing events. Promotes exon skipping. Cooperates with PTBP1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA (By similarity).
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Raver1 is a multifunctional protein that modulates both alternative splicing and focal adhesion assembly by binding to the nucleoplasmic splicing repressor polypyrimidine tract protein (PTB) or to the cytoskeletal proteins vinculin and alpha-actinin. The amino-terminal region of raver1 has three RNA recognition motif (RRM1, RRM2, and RRM3) domains, and RRM1 interacts with the vinculin tail (Vt) domain and vinculin mRNA. We previously determined the crystal structure of the raver1 RRM1-3 domains in complex with Vt at 2.75 A resolution. Here, we report crystal structure of the unbound raver1 RRM1-3 domains at 2 A resolution. The apo structure reveals that a bound sulfate ion disrupts an electrostatic interaction between the RRM1 and RRM2 domains, triggering a large relative domain movement of over 30 degrees . Superposition with other RNA-bound RRM structures places the sulfate ion near the superposed RNA phosphate group suggesting that this is the raver1 RNA binding site. While several single and some tandem RRM domain structures have been described, to the best of our knowledge, this is the second report of a three-tandem RRM domain structure.

Apo raver1 structure reveals distinct RRM domain orientations.,Rangarajan ES, Lee JH, Izard T Protein Sci. 2011 Jun 1. doi: 10.1002/pro.664. PMID:21633983<ref>PMID:21633983</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3smz" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Izard T]]
[[Category: Lee JH]]
[[Category: Rangarajan ES]]