View source for 3tlg


==Microcin C7 self immunity protein MccF in the inactive mutant APO state==
<StructureSection load='3tlg' size='340' side='right'caption='[[3tlg]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3tlg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TLG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TLG FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4993&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tlg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tlg OCA], [https://pdbe.org/3tlg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tlg RCSB], [https://www.ebi.ac.uk/pdbsum/3tlg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tlg ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/MCCF_ECOLX MCCF_ECOLX] Involved in specific self-immunity to microcin C7.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Several classes of naturally occurring antimicrobials exert their antibiotic activity by specifically targeting aminoacyl-tRNA synthetases, validating these enzymes as drug targets. The aspartyl tRNA synthetase "Trojan horse" inhibitor microcin C7 (McC7) consists of a nonhydrolyzable aspartyl-adenylate conjugated to a hexapeptide carrier that facilitates active import into bacterial cells through an oligopeptide transport system. Subsequent proteolytic processing releases the toxic compound inside the cell. Producing strains of McC7 must protect themselves against autotoxicity that may result from premature processing. The mccF gene confers resistance against endogenous and exogenous McC7 by hydrolyzing the amide bond that connects the peptide and nucleotide moieties of McC7. We present here crystal structures of MccF, in complex with various ligands. The MccF structure is similar to that of dipeptide ld-carboxypeptidase, but with an additional loop proximal to the active site that serves as the primary determinant for recognition of adenylated substrates. Wild-type MccF only hydrolyzes the naturally occurring aspartyl phosphoramidate McC7 and synthetic peptidyl sulfamoyl adenylates that contain anionic side chains. We show that substitutions of two active site MccF residues result in a specificity switch toward aromatic aminoacyl-adenylate substrates. These results suggest how MccF-like enzymes may be used to avert various toxic aminoacyl-adenylates that accumulate during antibiotic biosynthesis or in normal metabolism of the cell.

Structure and function of a serine carboxypeptidase adapted for degradation of the protein synthesis antibiotic microcin C7.,Agarwal V, Tikhonov A, Metlitskaya A, Severinov K, Nair SK Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):4425-30. Epub 2012 Mar 2. PMID:22388748<ref>PMID:22388748</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3tlg" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Large Structures]]
[[Category: Agarwal V]]
[[Category: Nair SK]]