View source for 3u48


==From soil to structure: a novel dimeric family 3-beta-glucosidase isolated from compost using metagenomic analysis==
<StructureSection load='3u48' size='340' side='right'caption='[[3u48]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3u48]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Compost_metagenome Compost metagenome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U48 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3U48 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3u48 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u48 OCA], [https://pdbe.org/3u48 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3u48 RCSB], [https://www.ebi.ac.uk/pdbsum/3u48 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3u48 ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
A recent metagenomic analysis sequenced a switchgrass-adapted compost community to identify enzymes from microorganisms that were specifically adapted to switchgrass under thermophilic conditions. These enzymes are being examined as part of the pretreatment process for the production of "second-generation" biofuels. Among the enzymes discovered was JMB19063, a novel three-domain beta-glucosidase that belongs to the GH3 (glycoside hydrolase 3) family. Here, we report the structure of JMB19063 in complex with glucose and the catalytic variant D261N crystallized in the presence of cellopentaose. JMB19063 is first structure of a dimeric member of the GH3 family, and we demonstrate that dimerization is required for catalytic activity. Arg-587 and Phe-598 from the C-terminal domain of the opposing monomer are shown to interact with bound ligands in the D261N structure. Enzyme assays confirmed that these residues are absolutely essential for full catalytic activity.

From soil to structure, a novel dimeric beta-glucosidase belonging to glycoside hydrolase family 3 isolated from compost using metagenomic analysis.,McAndrew RP, Park JI, Heins RA, Reindl W, Friedland GD, D'haeseleer P, Northen T, Sale KL, Simmons BA, Adams PD J Biol Chem. 2013 May 24;288(21):14985-92. doi: 10.1074/jbc.M113.458356. Epub , 2013 Apr 11. PMID:23580647<ref>PMID:23580647</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3u48" style="background-color:#fffaf0;"></div>

==See Also==
*[[Beta-glucosidase 3D structures|Beta-glucosidase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Compost metagenome]]
[[Category: Large Structures]]
[[Category: Adams PD]]
[[Category: D'haeseleer P]]
[[Category: Friedland GD]]
[[Category: McAndrew RP]]
[[Category: Northen T]]
[[Category: Park JI]]
[[Category: Reindl W]]
[[Category: Sale KL]]
[[Category: Simmons BA]]