View source for 3ujt


==Structure of the Fab fragment of Ab-52, an antibody that binds the O-antigen of Francisella tularensis==
<StructureSection load='3ujt' size='340' side='right'caption='[[3ujt]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3ujt]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UJT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UJT FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ujt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ujt OCA], [https://pdbe.org/3ujt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ujt RCSB], [https://www.ebi.ac.uk/pdbsum/3ujt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ujt ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Francisella tularensis (Ft), the Gram-negative facultative intracellular bacterium that causes tularemia, is considered a biothreat because of its high infectivity and the high mortality rate of respiratory disease. The Ft lipopolysaccharide (Ft LPS) is thought to be a main protective antigen in mice and humans, and we have previously demonstrated the protective effect of the Ft LPS-specific monoclonal antibody Ab52 in a mouse model of respiratory tularemia. Immunochemical characterization has shown that the epitope recognized by Ab52 is contained within two internal repeat units of the O-polysaccharide [O-antigen (OAg)] of Ft LPS. To further localize the Ab52 epitope and understand the molecular interactions between the antibody and the saccharide, we determined the X-ray crystal structure of the Fab fragment of Ab52 and derived an antibody-antigen complex using molecular docking. The docked complex, refined through energy minimization, reveals an antigen binding site in the shape of a large canyon with a central pocket that accommodates a V-shaped epitope consisting of six sugar residues, alpha-d-GalpNAcAN(1--&gt;4)-alpha-d-GalpNAcAN(1--&gt;3)-beta-d-QuipNAc(1--&gt;2)-beta-d-Qu ip4NFm(1--&gt;4)-alpha-d-GalpNAcAN(1--&gt;4)-alpha-d-GalpNAcAN. These results inform the development of vaccines and immunotherapeutic/immunoprophylactic antibodies against Ft by suggesting a desired topology for binding of the antibody to internal epitopes of Ft LPS. This is the first report of an X-ray crystal structure of a monoclonal antibody that targets a protective Ft B cell epitope.

Structural Analysis of a Protective Epitope of the Francisella tularensis O-Polysaccharide.,Rynkiewicz MJ, Lu Z, Hui JH, Sharon J, Seaton BA Biochemistry. 2012 Jul 2. PMID:22747335<ref>PMID:22747335</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 3ujt" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Hui JH]]
[[Category: Lu Z]]
[[Category: Rynkiewicz MJ]]
[[Category: Seaton BA]]
[[Category: Sharon J]]