View source for 3uk9


==Galactose-specific lectin from Dolichos lablab==
<StructureSection load='3uk9' size='340' side='right'caption='[[3uk9]], [[Resolution|resolution]] 3.11&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3uk9]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Lablab_purpureus Lablab purpureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UK9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UK9 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.11&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uk9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uk9 OCA], [https://pdbe.org/3uk9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uk9 RCSB], [https://www.ebi.ac.uk/pdbsum/3uk9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uk9 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/LECA2_LABPU LECA2_LABPU] D-galactose-binding lectin.<ref>PMID:16125971</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Crystal structure analysis of a galactose-specific lectin from a leguminous food crop Dolichos lablab (Indian lablab beans) has been carried out to obtain insights into its quaternary association and lectin-carbohydrate interactions. The analysis led to the identification of adenine binding sites at the dimeric interfaces of the heterotetrameric lectin. Structural details of similar adenine binding were reported in only one legume lectin, Dolichos biflorus, before this study. Here, we present the structure of the galactose-binding D. lablab lectin at different pH values in the native form and in complex with galactose and adenine. This first structure report on this lectin also provides a high resolution atomic view of legume lectin-adenine interactions. The tetramer has two canonical and two DB58-like interfaces. The binding of adenine, a non-carbohydrate ligand, is found to occur at four hydrophobic sites at the core of the tetramer at the DB58-like dimeric interfaces and does not interfere with the carbohydrate-binding site. To support the crystallographic observations, the adenine binding was further quantified by carrying out isothermal calorimetric titration. By this method, we not only estimated the affinity of the lectin to adenine but also showed that adenine binds with negative cooperativity in solution.

Affinity of a galactose-specific legume lectin from Dolichos lablab to adenine revealed by X-ray cystallography.,Shetty KN, Latha VL, Rao RN, Nadimpalli SK, Suguna K IUBMB Life. 2013 Jul;65(7):633-44. doi: 10.1002/iub.1177. PMID:23794513<ref>PMID:23794513</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3uk9" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Lablab purpureus]]
[[Category: Large Structures]]
[[Category: Latha VL]]
[[Category: Nadimpalli SK]]
[[Category: Rao RN]]
[[Category: Shetty KN]]
[[Category: Suguna K]]