View source for 3wbh


==Structural characteristics of alkaline phosphatase from a moderately halophilic bacteria Halomonas sp.593==
<StructureSection load='3wbh' size='340' side='right'caption='[[3wbh]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3wbh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Halomonas_sp._#593 Halomonas sp. #593]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WBH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WBH FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wbh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wbh OCA], [https://pdbe.org/3wbh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wbh RCSB], [https://www.ebi.ac.uk/pdbsum/3wbh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wbh ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/B5BP20_9GAMM B5BP20_9GAMM] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Alkaline phosphatase (AP) from the moderate halophilic bacterium Halomonas sp. 593 (HaAP) catalyzes the hydrolysis of phosphomonoesters over a wide salt-concentration range (1-4 M NaCl). In order to clarify the structural basis of its halophilic characteristics and its wide-range adaptation to salt concentration, the tertiary structure of HaAP was determined by X-ray crystallography to 2.1 A resolution. The unit cell of HaAP contained one dimer unit corresponding to the biological unit. The monomer structure of HaAP contains a domain comprised of an 11-stranded beta-sheet core with 19 surrounding alpha-helices similar to those of APs from other species, and a unique `crown' domain containing an extended `arm' structure that participates in formation of a hydrophobic cluster at the entrance to the substrate-binding site. The HaAP structure also displays a unique distribution of negatively charged residues and hydrophobic residues in comparison to other known AP structures. AP from Vibrio sp. G15-21 (VAP; a slight halophile) has the highest similarity in sequence (70.0% identity) and structure (C(alpha) r.m.s.d. of 0.82 A for the monomer) to HaAP. The surface of the HaAP dimer is substantially more acidic than that of the VAP dimer (144 exposed Asp/Glu residues versus 114, respectively), and thus may enable the solubility of HaAP under high-salt conditions. Conversely, the monomer unit of HaAP formed a substantially larger hydrophobic interior comprising 329 C atoms from completely buried residues, whereas that of VAP comprised 264 C atoms, which may maintain the stability of HaAP under low-salt conditions. These characteristics of HaAP may be responsible for its unique functional adaptation permitting activity over a wide range of salt concentrations.

Structural characteristics of alkaline phosphatase from the moderately halophilic bacterium Halomonas sp. 593.,Arai S, Yonezawa Y, Ishibashi M, Matsumoto F, Adachi M, Tamada T, Tokunaga H, Blaber M, Tokunaga M, Kuroki R Acta Crystallogr D Biol Crystallogr. 2014 Mar;70(Pt 3):811-20. doi:, 10.1107/S1399004713033609. Epub 2014 Feb 22. PMID:24598750<ref>PMID:24598750</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3wbh" style="background-color:#fffaf0;"></div>

==See Also==
*[[Alkaline phosphatase 3D structures|Alkaline phosphatase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Halomonas sp. #593]]
[[Category: Large Structures]]
[[Category: Arai S]]
[[Category: Ishibashi M]]
[[Category: Kuroki R]]
[[Category: Matsumoto F]]
[[Category: Tamada T]]
[[Category: Tokunaga H]]
[[Category: Tokunaga M]]
[[Category: Yonezawa Y]]