View source for 3wc3


==Crystal structure of endo-1,4-beta-glucanase from Eisenia fetida==
<StructureSection load='3wc3' size='340' side='right'caption='[[3wc3]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3wc3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Eisenia_fetida Eisenia fetida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WC3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WC3 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wc3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wc3 OCA], [https://pdbe.org/3wc3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wc3 RCSB], [https://www.ebi.ac.uk/pdbsum/3wc3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wc3 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/I2FI81_EISFE I2FI81_EISFE] 
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== Publication Abstract from PubMed ==
The saccharification process is essential for bioethanol production from woody biomass including celluloses. Cold-adapted cellulase, which has sufficient activity at low temperature (&lt;293 K), is capable of reducing heating costs during the saccharification process and is suitable for simultaneous saccharification and fermentation. Endo-1,4-beta-glucanase from the earthworm Eisenia fetida (EF-EG2) belonging to glycoside hydrolase family 9 has been shown to have the highest activity at 313 K, and also retained a comparatively high activity at 283 K. The recombinant EF-EG2 was purified expressed in Pichia pastoris, and then grew needle-shaped crystals with dimensions of 0.02 x 0.02 x 1 mm. The crystals belonged to the space group P3221 with unit-cell parameters of a = b = 136 A, c = 55.0 A. The final model of EF-EG2, including 435 residues, two ions, seven crystallization reagents and 696 waters, was refined to a crystallographic R-factor of 14.7% (free R-factor of 16.8%) to 1.5 A resolution. The overall structure of EF-EG2 has an (alpha/alpha)6 barrel fold which contains a putative active-site cleft and a negatively charged surface. This structural information helps us understand the catalytic and cold adaptation mechanisms of EF-EG2.

Crystal structure of endo-1,4-beta-glucanase from Eisenia fetida.,Arimori T, Ito A, Nakazawa M, Ueda M, Tamada T J Synchrotron Radiat. 2013 Nov;20(Pt 6):884-9. doi: 10.1107/S0909049513021110., Epub 2013 Oct 1. PMID:24121333<ref>PMID:24121333</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3wc3" style="background-color:#fffaf0;"></div>

==See Also==
*[[Glucanase 3D structures|Glucanase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Eisenia fetida]]
[[Category: Large Structures]]
[[Category: Arimori T]]
[[Category: Tamada T]]