View source for 3wia


==Crystal structure of the N-terminal 1-37 residues deleted mutant of Geobacillus copper nitrite reductase==
<StructureSection load='3wia' size='340' side='right'caption='[[3wia]], [[Resolution|resolution]] 1.77&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3wia]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_kaustophilus_HTA426 Geobacillus kaustophilus HTA426]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WIA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WIA FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.77&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wia FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wia OCA], [https://pdbe.org/3wia PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wia RCSB], [https://www.ebi.ac.uk/pdbsum/3wia PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wia ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q5L1X8_GEOKA Q5L1X8_GEOKA] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The crystal structures of copper-containing nitrite reductase (CuNiR) from the thermophilic Gram-positive bacterium Geobacillus kaustophilus HTA426 and the amino (N)-terminal 68 residue-deleted mutant were determined at resolutions of 1.3A and 1.8A, respectively. Both structures show a striking resemblance with the overall structure of the well-known CuNiRs composed of two Greek key beta-barrel domains; however, a remarkable structural difference was found in the N-terminal region. The unique region has one beta-strand and one alpha-helix extended to the northern surface of the type-1 copper site. The superposition of the Geobacillus CuNiR model on the electron-transfer complex structure of CuNiR with the redox partner cytochrome c551 in other denitrifier system led us to infer that this region contributes to the transient binding with the partner protein during the interprotein electron transfer reaction in the Geobacillus system. Furthermore, electron-transfer kinetics experiments using N-terminal residue-deleted mutant and the redox partner, Geobacillus cytochrome c551, were carried out. These structural and kinetics studies demonstrate that the region is directly involved in the specific partner recognition.

Structural and functional characterization of the Geobacillus copper nitrite reductase: involvement of the unique N-terminal region in the interprotein electron transfer with its redox partner.,Fukuda Y, Koteishi H, Yoneda R, Tamada T, Takami H, Inoue T, Nojiri M Biochim Biophys Acta. 2014 Mar;1837(3):396-405. doi:, 10.1016/j.bbabio.2014.01.004. Epub 2014 Jan 14. PMID:24440558<ref>PMID:24440558</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3wia" style="background-color:#fffaf0;"></div>

==See Also==
*[[Nitrite reductase 3D structures|Nitrite reductase 3D structures]]
== References ==
<references/>
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</StructureSection>
[[Category: Geobacillus kaustophilus HTA426]]
[[Category: Large Structures]]
[[Category: Fukuda Y]]
[[Category: Nojiri M]]