View source for 3wkl


==The periplasmic PDZ tandem fragment of the RseP homologue from Aquifex aeolicus==
<StructureSection load='3wkl' size='340' side='right'caption='[[3wkl]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3wkl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WKL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WKL FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wkl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wkl OCA], [https://pdbe.org/3wkl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wkl RCSB], [https://www.ebi.ac.uk/pdbsum/3wkl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wkl ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Y1964_AQUAE Y1964_AQUAE] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
During the extracytoplasmic stress response in Escherichia coli, the intramembrane protease RseP cleaves the anti-sigma(E) protein RseA only after the membrane-anchored protease DegS truncates the periplasmic part of RseA that suppresses the action of RseP. Here we analyzed the three-dimensional structure of the two tandemly arranged PSD-95/Dlg/ZO-1 (PDZ) domains (PDZ tandem) present in the periplasmic region of RseP and revealed that the two putative ligand-binding grooves constitute a single pocket-like structure that would lie just above the active center sequestrated within the membrane. Complete removal of the PDZ tandem from RseP led to the intramembrane cleavage of RseA without prior truncation by DegS. Furthermore, mutations expected to destabilize the tertiary structure of the PDZ tandem also caused the deregulation of the sequential cleavage. These observations suggest that the PDZ tandem serves as a size-exclusion filter to accommodate the truncated form of RseA into the active center.

A Structure-Based Model of Substrate Discrimination by a Noncanonical PDZ Tandem in the Intramembrane-Cleaving Protease RseP.,Hizukuri Y, Oda T, Tabata S, Tamura-Kawakami K, Oi R, Sato M, Takagi J, Akiyama Y, Nogi T Structure. 2014 Feb 4;22(2):326-36. doi: 10.1016/j.str.2013.12.003. Epub 2014 Jan, 2. PMID:24389025<ref>PMID:24389025</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3wkl" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Aquifex aeolicus]]
[[Category: Large Structures]]
[[Category: Nogi T]]
[[Category: Tabata S]]
[[Category: Takagi J]]
[[Category: Tamura-kawakami K]]