View source for 3wmu


==The structure of an anti-cancer lectin mytilec apo-form from the mussel Mytilus galloprovincialis==
<StructureSection load='3wmu' size='340' side='right'caption='[[3wmu]], [[Resolution|resolution]] 1.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3wmu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mytilus_galloprovincialis Mytilus galloprovincialis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WMU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WMU FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wmu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wmu OCA], [https://pdbe.org/3wmu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wmu RCSB], [https://www.ebi.ac.uk/pdbsum/3wmu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wmu ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/LEC1_MYTGA LEC1_MYTGA] Alpha-D-galactose-binding lectin (PubMed:27187419, PubMed:23093409, PubMed:27321048). Binds D-GalNAc, but not glucose or its derivatives (PubMed:27321048). Has hemagglutinating activity towards rabbit erythrocytes (PubMed:23093409, PubMed:27321048). Agglutinates bacteria. Has bacteriostatic activity on both Gram-positive and Gram-negative bacteria including B.subtilis, S.aureus, E.coli and V.parahaemolyticus, respectively (PubMed:27187419). Has a dose-dependent cytotoxic effect on the human globotriaosylceramide (Gb3)-expressing Epstein-Barr virus (EBV)-positive Burkitt's lymphoma (Raji) cell line (PubMed:23093409, PubMed:27321048). Has dose-dependent cytotoxic effect on another Burkitt's lymphoma (Ramos) cell line, which does not possess the EBV genome, but also expresses Gb3. Binds to Gb3 in these cells leading to phosphorylation of MEK1/2, ERK1/2, JNK and p38 kinase, activation of caspase-9/3 and to expression of p21 and tumor necrosis factor (TNF)-alpha (PubMed:26694420). No cytotoxic effect on the human chronic myelogenous leukemia (K-562) cell line, which does not express Gb3 (PubMed:23093409, PubMed:26694420). May be involved in innate immunity acting as an antibacterial or antifungal agent (PubMed:26694420, PubMed:27321048). May be a pattern recognition receptor (PRR) involved in recognition of glycans found on parasitic or symbiotic microorganisms (PubMed:27187419).<ref>PMID:23093409</ref> <ref>PMID:26694420</ref> <ref>PMID:27187419</ref> <ref>PMID:27321048</ref> 
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mytilus galloprovincialis]]
[[Category: Kawai F]]
[[Category: Noguchi H]]
[[Category: Ozeki Y]]
[[Category: Park S-Y]]
[[Category: Tame JRH]]
[[Category: Terada D]]
[[Category: Unzai S]]