View source for 3wmv


==The structure of an anti-cancer lectin mytilec with ligand from the mussel Mytilus galloprovincialis==
<StructureSection load='3wmv' size='340' side='right'caption='[[3wmv]], [[Resolution|resolution]] 1.05&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3wmv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mytilus_galloprovincialis Mytilus galloprovincialis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WMV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WMV FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.05&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A2G:N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE'>A2G</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wmv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wmv OCA], [https://pdbe.org/3wmv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wmv RCSB], [https://www.ebi.ac.uk/pdbsum/3wmv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wmv ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/LEC1_MYTGA LEC1_MYTGA] Alpha-D-galactose-binding lectin (PubMed:27187419, PubMed:23093409, PubMed:27321048). Binds D-GalNAc, but not glucose or its derivatives (PubMed:27321048). Has hemagglutinating activity towards rabbit erythrocytes (PubMed:23093409, PubMed:27321048). Agglutinates bacteria. Has bacteriostatic activity on both Gram-positive and Gram-negative bacteria including B.subtilis, S.aureus, E.coli and V.parahaemolyticus, respectively (PubMed:27187419). Has a dose-dependent cytotoxic effect on the human globotriaosylceramide (Gb3)-expressing Epstein-Barr virus (EBV)-positive Burkitt's lymphoma (Raji) cell line (PubMed:23093409, PubMed:27321048). Has dose-dependent cytotoxic effect on another Burkitt's lymphoma (Ramos) cell line, which does not possess the EBV genome, but also expresses Gb3. Binds to Gb3 in these cells leading to phosphorylation of MEK1/2, ERK1/2, JNK and p38 kinase, activation of caspase-9/3 and to expression of p21 and tumor necrosis factor (TNF)-alpha (PubMed:26694420). No cytotoxic effect on the human chronic myelogenous leukemia (K-562) cell line, which does not express Gb3 (PubMed:23093409, PubMed:26694420). May be involved in innate immunity acting as an antibacterial or antifungal agent (PubMed:26694420, PubMed:27321048). May be a pattern recognition receptor (PRR) involved in recognition of glycans found on parasitic or symbiotic microorganisms (PubMed:27187419).<ref>PMID:23093409</ref> <ref>PMID:26694420</ref> <ref>PMID:27187419</ref> <ref>PMID:27321048</ref> 
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mytilus galloprovincialis]]
[[Category: Kawai F]]
[[Category: Noguchi H]]
[[Category: Ozeki Y]]
[[Category: Park S-Y]]
[[Category: Tame JRH]]
[[Category: Terada D]]
[[Category: Unzai S]]