View source for 3wtp


==Crystal Structure of the heterotypic nucleosome containing human CENP-A and H3.3==
<StructureSection load='3wtp' size='340' side='right'caption='[[3wtp]], [[Resolution|resolution]] 2.67&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3wtp]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WTP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WTP FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.67&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wtp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wtp OCA], [https://pdbe.org/3wtp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wtp RCSB], [https://www.ebi.ac.uk/pdbsum/3wtp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wtp ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CENPA_HUMAN CENPA_HUMAN] Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division. The CENPA-H4 heterotetramer can bind DNA by itself (in vitro).<ref>PMID:20739937</ref> <ref>PMID:21478274</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The centromere-specific histone H3 variant, CENP-A, is overexpressed in particular aggressive cancer cells, where it can be mislocalized ectopically in the form of heterotypic nucleosomes containing H3.3. In the present study, we report the crystal structure of the heterotypic CENP-A/H3.3 particle and reveal its "hybrid structure", in which the physical characteristics of CENP-A and H3.3 are conserved independently within the same particle. The CENP-A/H3.3 nucleosome forms an unexpectedly stable structure as compared to the CENP-A nucleosome, and allows the binding of the essential centromeric protein, CENP-C, which is ectopically mislocalized in the chromosomes of CENP-A overexpressing cells.

Crystal structure and stable property of the cancer-associated heterotypic nucleosome containing CENP-A and H3.3.,Arimura Y, Shirayama K, Horikoshi N, Fujita R, Taguchi H, Kagawa W, Fukagawa T, Almouzni G, Kurumizaka H Sci Rep. 2014 Nov 19;4:7115. doi: 10.1038/srep07115. PMID:25408271<ref>PMID:25408271</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3wtp" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Almouzni G]]
[[Category: Arimura Y]]
[[Category: Fujita R]]
[[Category: Fukagawa T]]
[[Category: Horikoshi N]]
[[Category: Kagawa W]]
[[Category: Kurumizaka H]]
[[Category: Shirayama K]]