View source for 3wvq


==Structure of ATP grasp protein==
<StructureSection load='3wvq' size='340' side='right'caption='[[3wvq]], [[Resolution|resolution]] 1.96&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3wvq]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_cirratus Streptomyces cirratus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WVQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WVQ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.955&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wvq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wvq OCA], [https://pdbe.org/3wvq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wvq RCSB], [https://www.ebi.ac.uk/pdbsum/3wvq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wvq ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A0A6YVN3_9ACTN A0A0A6YVN3_9ACTN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Peptide antibiotics are typically biosynthesized by one of two distinct machineries in a ribosome-dependent or ribosome-independent manner. Pheganomycin (PGM (1)) and related analogs consist of the nonproteinogenic amino acid (S)-2-(3,5-dihydroxy-4-hydroxymethyl)phenyl-2-guanidinoacetic acid (2) and a proteinogenic core peptide, making their origin uncertain. We report the identification of the biosynthetic gene cluster from Streptomyces cirratus responsible for PGM production. Unexpectedly, the cluster contains a gene encoding multiple precursor peptides along with several genes plausibly encoding enzymes for the synthesis of amino acid 2. We identified PGM1, which has an ATP-grasp domain, as potentially capable of linking the precursor peptides with 2, and validate this hypothesis using deletion mutants and in vitro reconstitution. We document PGM1's substrate permissivity, which could be rationalized by a large binding pocket as confirmed via structural and mutagenesis experiments. This is to our knowledge the first example of cooperative peptide synthesis achieved by ribosomes and peptide ligases using a peptide nucleophile.

A peptide ligase and the ribosome cooperate to synthesize the peptide pheganomycin.,Noike M, Matsui T, Ooya K, Sasaki I, Ohtaki S, Hamano Y, Maruyama C, Ishikawa J, Satoh Y, Ito H, Morita H, Dairi T Nat Chem Biol. 2015 Jan;11(1):71-6. doi: 10.1038/nchembio.1697. Epub 2014 Nov 24. PMID:25402768<ref>PMID:25402768</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3wvq" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptomyces cirratus]]
[[Category: Dairi T]]
[[Category: Hamano Y]]
[[Category: Ishikawa J]]
[[Category: Ito H]]
[[Category: Maruyama C]]
[[Category: Matsui T]]
[[Category: Morita H]]
[[Category: Noike M]]
[[Category: Ooya K]]
[[Category: Sasaki I]]
[[Category: Satoh Y]]