View source for 3wz3


==Structure of a periplasmic fragment of TraM==
<StructureSection load='3wz3' size='340' side='right'caption='[[3wz3]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3wz3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmid_R64 Plasmid R64]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WZ3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WZ3 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wz3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wz3 OCA], [https://pdbe.org/3wz3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wz3 RCSB], [https://www.ebi.ac.uk/pdbsum/3wz3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wz3 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9R2H2_9ZZZZ Q9R2H2_9ZZZZ] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The human pathogen Legionella pneumophila delivers a large array of the effector proteins into host cells using the Dot/Icm type IVB secretion system. Among the proteins composing the Dot/Icm system, an inner membrane protein DotI is known to be crucial for the secretion function but its structure and role in type IV secretion had not been elucidated. We report here the crystal structures of the periplasmic domains of DotI and its ortholog in the conjugation system of plasmid R64, TraM. These structures reveal a striking similarity to VirB8, a component of type IVA secretion systems, suggesting that DotI/TraM is the type IVB counterpart of VirB8. We further show that DotI and its partial paralog DotJ form a stable heterocomplex. R64 TraM, encoded by the conjugative plasmid lacking DotJ ortholog, forms a homo-hexamer. The DotI-DotJ complex is distinct from the core complex, which spans both inner and outer membranes to form a substrate conduit, and seems not to stably associate with the core complex. These results give insight into VirB8-family inner membrane proteins essential for type IV secretion and aid towards understanding the molecular basis of secretion systems essential for bacterial pathogenesis.

Molecular and structural analysis of Legionella DotI gives insights into an inner membrane complex essential for type IV secretion.,Kuroda T, Kubori T, Thanh Bui X, Hyakutake A, Uchida Y, Imada K, Nagai H Sci Rep. 2015 Jun 3;5:10912. doi: 10.1038/srep10912. PMID:26039110<ref>PMID:26039110</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3wz3" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Plasmid R64]]
[[Category: Imada K]]
[[Category: Kubori T]]
[[Category: Kuroda T]]
[[Category: Nagai H]]
[[Category: Uchida Y]]