View source for 3zef


==Crystal structure of Prp8:Aar2 complex: second crystal form at 3.1 Angstrom resolution==
<StructureSection load='3zef' size='340' side='right'caption='[[3zef]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3zef]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZEF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZEF FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zef OCA], [https://pdbe.org/3zef PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zef RCSB], [https://www.ebi.ac.uk/pdbsum/3zef PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zef ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/AAR2_YEAST AAR2_YEAST] Involved in splicing pre-mRNA of the A1 cistron and other genes that are important for cell growth.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The active centre of the spliceosome consists of an intricate network formed by U5, U2 and U6 small nuclear RNAs, and a pre-messenger-RNA substrate. Prp8, a component of the U5 small nuclear ribonucleoprotein particle, crosslinks extensively with this RNA catalytic core. Here we present the crystal structure of yeast Prp8 (residues 885-2413) in complex with Aar2, a U5 small nuclear ribonucleoprotein particle assembly factor. The structure reveals tightly associated domains of Prp8 resembling a bacterial group II intron reverse transcriptase and a type II restriction endonuclease. Suppressors of splice-site mutations, and an intron branch-point crosslink, map to a large cavity formed by the reverse transcriptase thumb, and the endonuclease-like and RNaseH-like domains. This cavity is large enough to accommodate the catalytic core of group II intron RNA. The structure provides crucial insights into the architecture of the spliceosome active site, and reinforces the notion that nuclear pre-mRNA splicing and group II intron splicing have a common origin.

Crystal structure of Prp8 reveals active site cavity of the spliceosome.,Galej WP, Oubridge C, Newman AJ, Nagai K Nature. 2013 Jan 31;493(7434):638-43. doi: 10.1038/nature11843. Epub 2013 Jan 23. PMID:23354046<ref>PMID:23354046</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3zef" style="background-color:#fffaf0;"></div>

==See Also==
*[[Pre-mRNA splicing factors 3D structures|Pre-mRNA splicing factors 3D structures]]
*[[Pre-mRNA-splicing factor|Pre-mRNA-splicing factor]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Galej WP]]
[[Category: Nagai K]]
[[Category: Newman AJ]]
[[Category: Oubridge C]]