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==The structure of F1-ATPase from Saccharomyces cerevisiae inhibited by its regulatory protein IF1==
<StructureSection load='3zia' size='340' side='right'caption='[[3zia]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3zia]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZIA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZIA FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zia FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zia OCA], [https://pdbe.org/3zia PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zia RCSB], [https://www.ebi.ac.uk/pdbsum/3zia PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zia ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ATPA_YEAST ATPA_YEAST] Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites (By similarity).
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== Publication Abstract from PubMed ==
The structure of F(1)-ATPase from Saccharomyces cerevisiae inhibited by the yeast IF(1) has been determined at 2.5 A resolution. The inhibitory region of IF(1) from residues 1 to 36 is entrapped between the C-terminal domains of the alpha(DP)- and beta(DP)-subunits in one of the three catalytic interfaces of the enzyme. Although the structure of the inhibited complex is similar to that of the bovine-inhibited complex, there are significant differences between the structures of the inhibitors and their detailed interactions with F(1)-ATPase. However, the most significant difference is in the nucleotide occupancy of the catalytic beta(E)-subunits. The nucleotide binding site in beta(E)-subunit in the yeast complex contains an ADP molecule without an accompanying magnesium ion, whereas it is unoccupied in the bovine complex. Thus, the structure provides further evidence of sequential product release, with the phosphate and the magnesium ion released before the ADP molecule.

The structure of F1-ATPase from Saccharomyces cerevisiae inhibited by its regulatory protein IF1.,Robinson GC, Bason JV, Montgomery MG, Fearnley IM, Mueller DM, Leslie AG, Walker JE Open Biol. 2013 Feb 13;3(2):120164. doi: 10.1098/rsob.120164. PMID:23407639<ref>PMID:23407639</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 3zia" style="background-color:#fffaf0;"></div>

==See Also==
*[[ATPase 3D structures|ATPase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Bason JV]]
[[Category: Fearnley IM]]
[[Category: Leslie AGW]]
[[Category: Montgomery MG]]
[[Category: Mueller DM]]
[[Category: Robinson GC]]
[[Category: Walker JE]]