View source for 3ziu


==Crystal structure of Mycoplasma mobile Leucyl-tRNA Synthetase with Leu-AMS in the active site==
<StructureSection load='3ziu' size='340' side='right'caption='[[3ziu]], [[Resolution|resolution]] 2.07&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3ziu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mesomycoplasma_mobile Mesomycoplasma mobile]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZIU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZIU FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.07&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=LSS:5-O-(L-LEUCYLSULFAMOYL)ADENOSINE'>LSS</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ziu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ziu OCA], [https://pdbe.org/3ziu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ziu RCSB], [https://www.ebi.ac.uk/pdbsum/3ziu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ziu ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q6KHA5_MYCMO Q6KHA5_MYCMO] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Mycoplasma leucyl-tRNA synthetases (LeuRSs) have been identified in which the connective polypeptide 1 (CP1) amino acid editing domain that clears mischarged tRNAs are missing (Mycoplasma mobile) or highly degenerate (Mycoplasma synoviae). Thus, these enzymes rely on a clearance pathway called pretransfer editing, which hydrolyzes misactivated aminoacyl-adenylate intermediate via a nebulous mechanism that has been controversial for decades. Even as the sole fidelity pathway for clearing amino acid selection errors in the pathogenic M. mobile, pretransfer editing is not robust enough to completely block mischarging of tRNA, resulting in codon ambiguity and statistical proteins. A high-resolution X-ray crystal structure shows that M. mobile LeuRS structurally overlaps with other LeuRS cores. However, when CP1 domains from different aminoacyl-tRNA synthetases and origins were fused to this common LeuRS core, surprisingly, pretransfer editing was enhanced. It is hypothesized that the CP1 domain evolved as a molecular rheostat to balance multiple functions. These include distal control of specificity and enzyme activity in the ancient canonical core, as well as providing a separate hydrolytic active site for clearing mischarged tRNA.

Leucyl-tRNA synthetase editing domain functions as a molecular rheostat to control codon ambiguity in Mycoplasma pathogens.,Li L, Palencia A, Lukk T, Li Z, Luthey-Schulten ZA, Cusack S, Martinis SA, Boniecki MT Proc Natl Acad Sci U S A. 2013 Mar 5;110(10):3817-22. doi:, 10.1073/pnas.1218374110. Epub 2013 Feb 19. PMID:23431144<ref>PMID:23431144</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3ziu" style="background-color:#fffaf0;"></div>

==See Also==
*[[Aminoacyl tRNA synthetase 3D structures|Aminoacyl tRNA synthetase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mesomycoplasma mobile]]
[[Category: Boniecki MT]]
[[Category: Cusack S]]
[[Category: Li L]]
[[Category: Li Z]]
[[Category: Lukk T]]
[[Category: Luthey-Schulten ZA]]
[[Category: Martinis SA]]
[[Category: Palencia A]]