View source for 3zj8


==Crystal structure of strictosidine glucosidase in complex with inhibitor-2==
<StructureSection load='3zj8' size='340' side='right'caption='[[3zj8]], [[Resolution|resolution]] 3.01&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3zj8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rauvolfia_serpentina Rauvolfia serpentina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZJ8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZJ8 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.01&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LR1:(1R,2S,3S,4R,5R)-4-[(4-BROMOPHENYL)METHYLAMINO]-5-(HYDROXYMETHYL)CYCLOPENTANE-1,2,3-TRIOL'>LR1</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zj8 OCA], [https://pdbe.org/3zj8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zj8 RCSB], [https://www.ebi.ac.uk/pdbsum/3zj8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zj8 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SG1_RAUSE SG1_RAUSE] Glucosidase specifically involved in alkaloid biosynthesis leading to the accumulation of several alkaloids, including ajmaline, an important plant-derived pharmaceutical used in the therapy of heart disorders.<ref>PMID:22004291</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Abstract Insight into the structure and inhibition mechanism of O-beta-d-glucosidases by deoxa-pyranosylamine type inhibitors is provided by X-ray analysis of complexes between raucaffricine and strictosidine glucosidases and N-(cyclohexylmethyl)-, N-(cyclohexyl)- and N-(bromobenzyl)-beta-d-gluco-1,5-deoxa-pyranosylamine. All inhibitors anchored exclusively in the catalytic active site by competition with appropriate enzyme substrates. Thus facilitated prospective elucidation of the binding networks with residues located at &lt;3.9 A distance will enable the development of potent inhibitors suitable for the production of valuable alkaloid glucosides, raucaffricine and strictosidine, by means of synthesis in Rauvolfia serpentina cell suspension cultures.

Ligand structures of synthetic deoxa-pyranosylamines with raucaffricine and strictosidine glucosidases provide structural insights into their binding and inhibitory behaviours.,Xia L, Lin H, Staniek A, Panjikar S, Ruppert M, Hilgers P, Williardt J, Rajendran C, Wang M, Warzecha H, Jager V, Stockigt J J Enzyme Inhib Med Chem. 2014 Aug 20:1-7. PMID:25140865<ref>PMID:25140865</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3zj8" style="background-color:#fffaf0;"></div>

==See Also==
*[[Beta-glucosidase 3D structures|Beta-glucosidase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Rauvolfia serpentina]]
[[Category: Castiglia A]]
[[Category: Jaeger V]]
[[Category: Lin H]]
[[Category: Panjikar S]]
[[Category: Rajendran C]]
[[Category: Ruppert M]]
[[Category: Schuebel H]]
[[Category: Stoeckigt J]]
[[Category: Wang M]]
[[Category: Warzecha H]]
[[Category: Xia L]]