View source for 3zl5


==Crystal structure of Schistosoma mansoni Peroxiredoxin I C48S mutant with one decamer in the ASU==
<StructureSection load='3zl5' size='340' side='right'caption='[[3zl5]], [[Resolution|resolution]] 2.49&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3zl5]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Schistosoma_mansoni Schistosoma mansoni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZL5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZL5 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.493&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zl5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zl5 OCA], [https://pdbe.org/3zl5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zl5 RCSB], [https://www.ebi.ac.uk/pdbsum/3zl5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zl5 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/O97161_SCHMA O97161_SCHMA] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Members of the typical 2-Cys Peroxiredoxin (Prx) subfamily represent an intriguing example of protein moonlighting behavior, since this enzyme shifts function: indeed, upon chemical stimuli, such as oxidative stress, Prx undergoes a switch from peroxidase to molecular chaperone, associated to a change in quaternary structure from dimers/decamers to higher molecular weight (HMW) species. In order to detail at molecular level the structural mechanism of this switch, we have designed and expressed mutants of peroxiredoxin I from Schistosoma mansoni (SmPrxI) with constitutive HMW assembly and molecular chaperone activity. By a combination of X-ray crystallography, transmission electron microscopy and functional experiments we defined the structural events responsible for the moonlighting behavior of 2-Cys Prx and we demonstrated that acidification is coupled to local structural variations localized at the active site and a change in oligomerization to HMW forms, similar to those induced by oxidative stress. Moreover, we suggest that the binding site of the unfolded polypeptide is at least in part contributed by the hydrophobic surface exposed by the unfolding of the active site. We also find an inverse correlation between the extent of ring stacking and molecular chaperone activity which is explained assuming that the binding occurs at the extremities of the nanotube and the longer the nanotube, the lesser the ratio binding sites/molecular mass.

Switching between the alternative structures and functions of a 2-Cys Peroxiredoxin, by site-directed mutagenesis.,Angelucci F, Saccoccia F, Ardini M, Boumis G, Brunori M, Di Leandro L, Ippoliti R, Miele AE, Natoli G, Scotti S, Bellelli A J Mol Biol. 2013 Sep 7. pii: S0022-2836(13)00559-7. doi:, 10.1016/j.jmb.2013.09.002. PMID:24021815<ref>PMID:24021815</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3zl5" style="background-color:#fffaf0;"></div>

==See Also==
*[[Peroxiredoxin 3D structures|Peroxiredoxin 3D structures]]
*[[Thioredoxin 3D structures|Thioredoxin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Schistosoma mansoni]]
[[Category: Angelucci F]]
[[Category: Ardini M]]
[[Category: Bellelli A]]
[[Category: Boumis G]]
[[Category: Brunori M]]
[[Category: DiLeandro L]]
[[Category: Ippoliti R]]
[[Category: Miele AE]]
[[Category: Natoli G]]
[[Category: Saccoccia F]]
[[Category: Scotti S]]