View source for 3zlg


==Structure of group A Streptococcal enolase K362A mutant==
<StructureSection load='3zlg' size='340' side='right'caption='[[3zlg]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3zlg]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pyogenes_MGAS10394 Streptococcus pyogenes MGAS10394]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZLG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZLG FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zlg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zlg OCA], [https://pdbe.org/3zlg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zlg RCSB], [https://www.ebi.ac.uk/pdbsum/3zlg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zlg ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ENO_STRP6 ENO_STRP6] Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis. Binds plasminogen when expressed on the bacterial cell surface, potentially allowing the bacterium to acquire surface-associated proteolytic activity, which in turn contributes to tissue invasion and virulence.<ref>PMID:9603964</ref> 
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== Publication Abstract from PubMed ==
Group A Streptococcus (GAS) is a human pathogen that has the potential to cause invasive disease by binding and activating human plasmin(ogen). Streptococcal surface enolase (SEN) is an octameric alpha-enolase that is localized at the GAS cell surface. In addition to its glycolytic role inside the cell, SEN functions as a receptor for plasmin(ogen) on the bacterial surface, but the understanding of the molecular basis of plasmin(ogen) binding is limited. In this study, we determined the crystal and solution structures of GAS SEN and characterized the increased plasminogen binding by two SEN mutants. The plasminogen binding ability of SENK312A and SENK362A is ~2- and ~3.4-fold greater than for the wild-type protein. A combination of thermal stability assays, native mass spectrometry and X-ray crystallography approaches shows that increased plasminogen binding ability correlates with decreased stability of the octamer. We propose that decreased stability of the octameric structure facilitates the access of plasmin(ogen) to its binding sites, leading to more efficient plasmin(ogen) binding and activation.

Stability of the octameric structure affects plasminogen-binding capacity of streptococcal enolase.,Cork AJ, Ericsson DJ, Law RH, Casey LW, Valkov E, Bertozzi C, Stamp A, Jovcevski B, Aquilina JA, Whisstock JC, Walker MJ, Kobe B PLoS One. 2015 Mar 25;10(3):e0121764. doi: 10.1371/journal.pone.0121764., eCollection 2015. PMID:25807546<ref>PMID:25807546</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 3zlg" style="background-color:#fffaf0;"></div>

==See Also==
*[[Enolase 3D structures|Enolase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptococcus pyogenes MGAS10394]]
[[Category: Aquilina JA]]
[[Category: Bertozzi C]]
[[Category: Casey LW]]
[[Category: Cork AJ]]
[[Category: Ericsson DJ]]
[[Category: Kobe B]]
[[Category: Law RHP]]
[[Category: Stamp A]]
[[Category: Valkov E]]
[[Category: Walker MJ]]
[[Category: Whisstock JC]]