View source for 3zop


==Arg90Cit chorismate mutase of Bacillus subtilis at 1.6 A resolution==
<StructureSection load='3zop' size='340' side='right'caption='[[3zop]], [[Resolution|resolution]] 1.61&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3zop]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZOP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZOP FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.61&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIR:CITRULLINE'>CIR</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zop FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zop OCA], [https://pdbe.org/3zop PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zop RCSB], [https://www.ebi.ac.uk/pdbsum/3zop PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zop ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/AROH_BACSU AROH_BACSU] Catalyzes the Claisen rearrangement of chorismate to prephenate. Probably involved in the aromatic amino acid biosynthesis.<ref>PMID:2105742</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
For more than half a century, transition state theory has provided a useful framework for understanding the origins of enzyme catalysis. As proposed by Pauling, enzymes accelerate chemical reactions by binding transition states tighter than substrates, thereby lowering the activation energy compared with that of the corresponding uncatalyzed process. This paradigm has been challenged for chorismate mutase (CM), a well-characterized metabolic enzyme that catalyzes the rearrangement of chorismate to prephenate. Calculations have predicted the decisive factor in CM catalysis to be ground state destabilization rather than transition state stabilization. Using X-ray crystallography, we show, in contrast, that a sluggish variant of Bacillus subtilis CM, in which a cationic active-site arginine was replaced by a neutral citrulline, is a poor catalyst even though it effectively preorganizes chorismate for the reaction. A series of high-resolution molecular snapshots of the reaction coordinate, including the apo enzyme, and complexes with substrate, transition state analog and product, demonstrate that an active site, which is only complementary in shape to a reactive substrate conformer, is insufficient for effective catalysis. Instead, as with other enzymes, electrostatic stabilization of the CM transition state appears to be crucial for achieving high reaction rates.

Electrostatic transition state stabilization rather than reactant destabilization provides the chemical basis for efficient chorismate mutase catalysis.,Burschowsky D, van Eerde A, Okvist M, Kienhofer A, Kast P, Hilvert D, Krengel U Proc Natl Acad Sci U S A. 2014 Nov 24. pii: 201408512. PMID:25422475<ref>PMID:25422475</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3zop" style="background-color:#fffaf0;"></div>

==See Also==
*[[3D structures of chorismate mutase|3D structures of chorismate mutase]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bacillus subtilis]]
[[Category: Large Structures]]
[[Category: Burschowsky D]]
[[Category: Hilvert D]]
[[Category: Kast P]]
[[Category: Kienhofer A]]
[[Category: Krengel U]]
[[Category: Okvist M]]
[[Category: VanEerde A]]