View source for 3zqs


==Human FANCL central domain==
<StructureSection load='3zqs' size='340' side='right'caption='[[3zqs]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3zqs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZQS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZQS FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=PRO:PROLINE'>PRO</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zqs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zqs OCA], [https://pdbe.org/3zqs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zqs RCSB], [https://www.ebi.ac.uk/pdbsum/3zqs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zqs ProSAT]</span></td></tr>
</table>
== Disease ==
[https://www.uniprot.org/uniprot/FANCL_HUMAN FANCL_HUMAN] Fanconi anemia. The disease is caused by mutations affecting the gene represented in this entry.
== Function ==
[https://www.uniprot.org/uniprot/FANCL_HUMAN FANCL_HUMAN] Ubiquitin ligase protein that mediates monoubiquitination of FANCD2, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCI. May stimulate the ubiquitin release from UBE2W. May be required for proper primordial germ cell proliferation in the embryonic stage, whereas it is probably not needed for spermatogonial proliferation after birth.<ref>PMID:12973351</ref> <ref>PMID:16916645</ref> <ref>PMID:17938197</ref> <ref>PMID:19111657</ref> <ref>PMID:19589784</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The Fanconi Anemia (FA) pathway is essential for the repair of DNA interstrand cross-links. At the heart of this pathway is the monoubiquitination of the FANCI/FANCD2 (ID) complex by the multiprotein 'core complex' containing the E3 ubiquitin ligase FANCL. Vertebrate organisms have the 8-protein core complex, while invertebrates apparently do not. We report here the structure of the central domain of human FANCL in comparison with the recently solved Drosophila melanogaster FANCL. Our data represent the first structural detail into the catalytic core of the human system, and reveal that the central fold of FANCL is conserved between species. However, there are macromolecular differences between the FANCL proteins that may account for the apparent distinctions in core complex requirements between the vertebrate and invertebrate FA pathways. In addition we characterize the binding of human FANCL with its partners, Ube2t, FANCD2 and FANCI. Mutational analysis reveals, which residues are required for substrate binding, and we also show the domain required for E2 binding.

Structural analysis of human FANCL, the E3 ligase in the fanconi anemia pathway.,Hodson C, Cole AR, Lewis LP, Miles JA, Purkiss-Trew A, Walden H J Biol Chem. 2011 Jul 20. PMID:21775430<ref>PMID:21775430</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3zqs" style="background-color:#fffaf0;"></div>

==See Also==
*[[Ubiquitin protein ligase 3D structures|Ubiquitin protein ligase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Cole AR]]
[[Category: Hodson C]]
[[Category: Purkiss-Trew A]]
[[Category: Walden H]]