View source for 3zr8


==Crystal structure of RxLR effector Avr3a11 from Phytophthora capsici==
<StructureSection load='3zr8' size='340' side='right'caption='[[3zr8]], [[Resolution|resolution]] 0.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3zr8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Phytophthora_capsici Phytophthora capsici]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZR8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZR8 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 0.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zr8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zr8 OCA], [https://pdbe.org/3zr8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zr8 RCSB], [https://www.ebi.ac.uk/pdbsum/3zr8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zr8 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/G1K3S4_PHYCP G1K3S4_PHYCP] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Phytopathogens deliver effector proteins inside host plant cells to promote infection. These proteins can also be sensed by the plant immune system, leading to restriction of pathogen growth. Effector genes can display signatures of positive selection and rapid evolution, presumably a consequence of their co-evolutionary arms race with plants. The molecular mechanisms underlying how effectors evolve to gain new virulence functions and/or evade the plant immune system are poorly understood. Here, we report the crystal structures of the effector domains from two oomycete RXLR proteins, Phytophthora capsici AVR3a11 and Phytophthora infestans PexRD2. Despite sharing &lt;20% sequence identity in their effector domains, they display a conserved core alpha-helical fold. Bioinformatic analyses suggest that the core fold occurs in approximately 44% of annotated Phytophthora RXLR effectors, both as a single domain and in tandem repeats of up to 11 units. Functionally important and polymorphic residues map to the surface of the structures, and PexRD2, but not AVR3a11, oligomerizes in planta. We conclude that the core alpha-helical fold enables functional adaptation of these fast evolving effectors through (i) insertion/deletions in loop regions between alpha-helices, (ii) extensions to the N and C termini, (iii) amino acid replacements in surface residues, (iv) tandem domain duplications, and (v) oligomerization. We hypothesize that the molecular stability provided by this core fold, combined with considerable potential for plasticity, underlies the evolution of effectors that maintain their virulence activities while evading recognition by the plant immune system.

Structures of Phytophthora RXLR Effector Proteins: A CONSERVED BUT ADAPTABLE FOLD UNDERPINS FUNCTIONAL DIVERSITY.,Boutemy LS, King SR, Win J, Hughes RK, Clarke TA, Blumenschein TM, Kamoun S, Banfield MJ J Biol Chem. 2011 Oct 14;286(41):35834-42. Epub 2011 Aug 3. PMID:21813644<ref>PMID:21813644</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3zr8" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Phytophthora capsici]]
[[Category: Banfield MJ]]
[[Category: Blumenschein TMA]]
[[Category: Boutemy LS]]
[[Category: Clarke TA]]
[[Category: Hughes RK]]
[[Category: Kamoun S]]
[[Category: King SRF]]
[[Category: Win J]]