View source for 3ztg


==Solution structure of the RING finger-like domain of Retinoblastoma Binding Protein-6 (RBBP6)==
<StructureSection load='3ztg' size='340' side='right'caption='[[3ztg]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3ztg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZTG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZTG FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ztg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ztg OCA], [https://pdbe.org/3ztg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ztg RCSB], [https://www.ebi.ac.uk/pdbsum/3ztg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ztg ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RBBP6_HUMAN RBBP6_HUMAN] E3 ubiquitin-protein ligase which promotes ubiquitination of YBX1, leading to its degradation by the proteasome. May play a role as a scaffold protein to promote the assembly of the p53/TP53-MDM2 complex, resulting in increase of MDM2-mediated ubiquitination and degradation of p53/TP53; may function as negative regulator of p53/TP53, leading to both apoptosis and cell growth.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Retinoblastoma-binding protein-6 (RBBP6) plays a facilitating role, through its RING finger-like domain, in the ubiquitination of p53 by Hdm2 that is suggestive of E4-like activity. Although the presence of eight conserved cysteine residues makes it highly probable that the RING finger-like domain coordinates two zinc ions, analysis of the primary sequence suggests an alternative classification as a member of the U-box family, the members of which do not bind zinc ions. We show here that despite binding two zinc ions, the domain adopts a homodimeric structure highly similar to those of a number of U-boxes. Zinc ions could be replaced by cadmium ions without significantly disrupting the structure or the stability of the domain, although the rate of substitution was an order of magnitude slower than any previous measurement, suggesting that the structure is particularly stable, a conclusion supported by the high thermal stability of the domain. A hallmark of U-box-containing proteins is their association with chaperones, with which they cooperate in eliminating irretrievably unfolded proteins by tagging them for degradation by the proteasome. Using a yeast two-hybrid screen, we show that RBBP6 interacts with chaperones Hsp70 and Hsp40 through its N-terminal ubiquitin-like domain. Taken together with the structural similarities to U-box-containing proteins, our data suggest that RBBP6 plays a role in chaperone-mediated ubiquitination and possibly in protein quality control.

Solution Structure of RING Finger-like Domain of Retinoblastoma-binding Protein-6 (RBBP6) Suggests It Functions as a U-box.,Kappo MA, Ab E, Hassem F, Atkinson RA, Faro A, Muleya V, Mulaudzi T, Poole JO, McKenzie JM, Chibi M, Moolman-Smook JC, Rees DJ, Pugh DJ J Biol Chem. 2012 Mar 2;287(10):7146-58. Epub 2011 Nov 29. PMID:22130672<ref>PMID:22130672</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3ztg" style="background-color:#fffaf0;"></div>

==See Also==
*[[Retinoblastoma-binding protein 3D structures|Retinoblastoma-binding protein 3D structures]]
*[[Ubiquitin protein ligase 3D structures|Ubiquitin protein ligase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Ab E]]
[[Category: Atkinson RA]]
[[Category: Faro A]]
[[Category: Kappo MA]]
[[Category: McKenzie JM]]
[[Category: Mulaudzi T]]
[[Category: Muleya V]]
[[Category: Poole JO]]
[[Category: Pugh DJR]]