View source for 3zv0


==Structure of the SHQ1P-CBF5P complex==
<StructureSection load='3zv0' size='340' side='right'caption='[[3zv0]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3zv0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZV0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZV0 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zv0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zv0 OCA], [https://pdbe.org/3zv0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zv0 RCSB], [https://www.ebi.ac.uk/pdbsum/3zv0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zv0 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SHQ1_YEAST SHQ1_YEAST] Involved in the early biogenesis steps of box H/ACA snoRNP assembly.<ref>PMID:12228251</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
SHQ1 is an essential assembly factor for H/ACA ribonucleoproteins (RNPs) required for ribosome biogenesis, pre-mRNA splicing, and telomere maintenance. SHQ1 binds dyskerin/NAP57, the catalytic subunit of human H/ACA RNPs, and this interaction is modulated by mutations causing X-linked dyskeratosis congenita. We report the crystal structure of the C-terminal domain of yeast SHQ1, Shq1p, and its complex with yeast dyskerin/NAP57, Cbf5p, lacking its catalytic domain. The C-terminal domain of Shq1p interacts with the RNA-binding domain of Cbf5p and, through structural mimicry, uses the RNA-protein-binding sites to achieve a specific protein-protein interface. We propose that Shq1p operates as a Cbf5p chaperone during RNP assembly by acting as an RNA placeholder, thereby preventing Cbf5p from nonspecific RNA binding before association with an H/ACA RNA and the other core RNP proteins.

The H/ACA RNP assembly factor SHQ1 functions as an RNA mimic.,Walbott H, Machado-Pinilla R, Liger D, Blaud M, Rety S, Grozdanov PN, Godin K, van Tilbeurgh H, Varani G, Meier UT, Leulliot N Genes Dev. 2011 Nov 15;25(22):2398-408. PMID:22085966<ref>PMID:22085966</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3zv0" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae S288C]]
[[Category: Blaud M]]
[[Category: Godin K]]
[[Category: Grozdanov PN]]
[[Category: Leulliot N]]
[[Category: Liger D]]
[[Category: Machado-Pinilla R]]
[[Category: Meier UT]]
[[Category: Rety S]]
[[Category: Varani G]]
[[Category: Walbott H]]
[[Category: VanTilbeurgh H]]