View source for 3zvs


==Crystal structure of Archaemetzincin (AmzA) from Archaeoglobus fulgidus at 1.4 A resolution complexed with malonate==
<StructureSection load='3zvs' size='340' side='right'caption='[[3zvs]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3zvs]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZVS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZVS FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.396&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zvs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zvs OCA], [https://pdbe.org/3zvs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zvs RCSB], [https://www.ebi.ac.uk/pdbsum/3zvs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zvs ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/AMZA_ARCFU AMZA_ARCFU] Probable zinc metalloprotease whose natural substrate is unknown (By similarity).[HAMAP-Rule:MF_01842]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
BACKGROUND: Archaemetzincins are metalloproteases occurring in archaea and some mammalia. They are distinct from all the other metzincins by their extended active site consensus sequence HEXXHXXGXXHCX(4)CXMX(17)CXXC featuring four conserved cysteine residues. Very little is known about their biological importance and structure-function relationships. PRINCIPAL FINDINGS: Here we present three crystal structures of the archaemetzincin AfAmzA (Uniprot O29917) from Archaeoglobus fulgidus, revealing a metzincin architecture featuring a zinc finger-like structural element involving the conserved cysteines of the consensus motif. The active sites in all three structures are occluded to different extents rendering the enzymes proteolytically inactive against a large variety of tested substrates. Owing to the different ligand binding there are significant differences in active site architecture, revealing a large flexibility of the loops covering the active site cleft. CONCLUSIONS: The crystal structures of AfAmzA provide the structural basis for the lack of activity in standard proteolytic assays and imply a triggered activity onset upon opening of the active site cleft.

Crystal structures of archaemetzincin reveal a moldable substrate-binding site.,Graef C, Schacherl M, Waltersperger S, Baumann U PLoS One. 2012;7(8):e43863. Epub 2012 Aug 24. PMID:22937112<ref>PMID:22937112</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3zvs" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Archaeoglobus fulgidus]]
[[Category: Large Structures]]
[[Category: Baumann U]]
[[Category: Graef C]]
[[Category: Schacherl M]]