View source for 3zzm


==Crystal structure of Mycobacterium tuberculosis PurH with a novel bound nucleotide CFAIR, at 2.2 A resolution.==
<StructureSection load='3zzm' size='340' side='right'caption='[[3zzm]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3zzm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZZM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZZM FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=JLN:5-(FORMYLAMINO)-1-(5-O-PHOSPHONO-BETA-D-RIBOFURANOSYL)-1H-IMIDAZOLE-4-CARBOXYLIC+ACID'>JLN</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zzm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zzm OCA], [https://pdbe.org/3zzm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zzm RCSB], [https://www.ebi.ac.uk/pdbsum/3zzm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zzm ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PUR9_MYCTU PUR9_MYCTU] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Enzymes of the de novo purine biosynthetic pathway have been identified as essential for the growth and survival of Mycobacterium tuberculosis (Mtb), and thus have potential for the development of anti-TB drugs. The final two steps of this pathway are carried out by the bifunctional enzyme 5-aminoimidazole-4-carboxamide ribonucleotide transformylase /inosine monophosphate cyclohydrolase (ATIC), also known as PurH. This enzyme has already been the target of anti-cancer drug development. We have determined crystal structures of the Mtb ATIC (Rv0957) both with and without the substrate AICAR, at resolutions of 2.5 A and 2.2 A, respectively. As for other ATIC enzymes, the protein is folded into two domains, the N-terminal domain (residues 1-212) containing the cyclohydrolase active site and the C-terminal domain (residues 222-523) the formyl transferase active site. An adventitiously-bound nucleotide was found in the cyclohydrolase active site in both structures, and was identified by NMR and mass spectral analysis as a novel 5-formyl derivative of an earlier intermediate in the biosynthetic pathway, 4-carboxy-5-aminoimidazole ribonucleotide (CAIR). This result and other studies suggest that this novel nucleotide is a cyclohydrolase inhibitor. The dimer formed by Mtb ATIC is different from those seen for human and avian ATICs, but has a similar ~ 50 A separation of the two active sites of the bifunctional enzyme. Evidence in Mtb ATIC for half-the-sites reactivity in the cyclohydrolase domains can be attributed to ligand-induced movements that propagate across the dimer interface and may be a common feature of ATIC enzymes.

Structural analyses of a purine biosynthetic enzyme from Mycobacterium tuberculosis reveal a novel bound nucleotide.,Le Nours J, Bulloch EM, Zhang Z, Greenwood DR, Middleditch MJ, Dickson JM, Baker EN J Biol Chem. 2011 Sep 28. PMID:21956117<ref>PMID:21956117</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3zzm" style="background-color:#fffaf0;"></div>

==See Also==
*[[Bifunctional purine biosynthesis protein PURH|Bifunctional purine biosynthesis protein PURH]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mycobacterium tuberculosis H37Rv]]
[[Category: Baker EN]]
[[Category: Bulloch EMM]]
[[Category: Dickson JMJ]]
[[Category: Greenwood DR]]
[[Category: Le Nours J]]
[[Category: Middleditch MJ]]
[[Category: Zhang Z]]