View source for 4a1w


==Crystal structure of alpha-beta foldamer 4c in complex with Bcl-xL==
<StructureSection load='4a1w' size='340' side='right'caption='[[4a1w]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4a1w]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A1W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4A1W FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.497&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3FB:(3S)-3-AMINO-4-PHENYLBUTANOIC+ACID'>3FB</scene>, <scene name='pdbligand=B3E:(3S)-3-AMINOHEXANEDIOIC+ACID'>B3E</scene>, <scene name='pdbligand=B3Y:(3S)-3-AMINO-4-(4-HYDROXYPHENYL)BUTANOIC+ACID'>B3Y</scene>, <scene name='pdbligand=BIL:(3R,4S)-3-AMINO-4-METHYLHEXANOIC+ACID'>BIL</scene>, <scene name='pdbligand=HT7:(3S)-3-AMINO-4-(1H-INDOL-3-YL)BUTANOIC+ACID'>HT7</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4a1w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a1w OCA], [https://pdbe.org/4a1w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4a1w RCSB], [https://www.ebi.ac.uk/pdbsum/4a1w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4a1w ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/B2CL1_HUMAN B2CL1_HUMAN] Potent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis.<ref>PMID:19917720</ref> <ref>PMID:21840391</ref>   Isoform Bcl-X(S) promotes apoptosis.<ref>PMID:19917720</ref> <ref>PMID:21840391</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Peptidic oligomers that contain both alpha- and beta-amino acid residues, in regular patterns throughout the backbone, are emerging as structural mimics of alpha-helix-forming conventional peptides (composed exclusively of alpha-amino acid residues). Here we describe a comprehensive evaluation of diverse alpha/beta-peptide homologues of the Bim BH3 domain in terms of their ability to bind to the BH3-recognition sites on two partner proteins, Bcl-x(L) and Mcl-1. These proteins are members of the anti-apoptotic Bcl-2 family, and both bind tightly to the Bim BH3 domain itself. All alpha/beta-peptide homologues retain the side-chain sequence of the Bim BH3 domain, but each homologue contains periodic alpha-residue --&gt; beta(3)-residue substitutions. Previous work has shown that the alphaalphabetaalphaalphaalphabeta pattern, which aligns the beta(3)-residues in a 'stripe' along one side of the helix, can support functional alpha-helix mimicry, and the results reported here strengthen this conclusion. The present study provides the first evaluation of functional mimicry by alphaalphabeta and alphaalphaalphabeta patterns, which cause the beta(3)-residues to spiral around the helix periphery. We find that the alphaalphaalphabeta pattern can support effective mimicry of the Bim BH3 domain, as manifested by the crystal structure of an alpha/beta-peptide bound to Bcl-x(L), affinity for a variety of Bcl-2 family proteins, and induction of apoptotic signaling in mouse embryonic fibroblast extracts. The best alphaalphaalphabeta homologue shows substantial protection from proteolytic degradation relative to the Bim BH3 alpha-peptide.

Evaluation of Diverse alpha/beta-Backbone Patterns for Functional alpha-Helix Mimicry: Analogues of the Bim BH3 Domain.,Boersma MD, Haase HS, Peterson-Kaufman KJ, Lee EF, Clarke OB, Colman PM, Smith BJ, Horne WS, Fairlie WD, Gellman SH J Am Chem Soc. 2011 Dec 8. PMID:22040025<ref>PMID:22040025</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4a1w" style="background-color:#fffaf0;"></div>

==See Also==
*[[B-cell lymphoma proteins 3D structures|B-cell lymphoma proteins 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Synthetic construct]]
[[Category: Boersma MD]]
[[Category: Clarke OB]]
[[Category: Colman PM]]
[[Category: Fairlie WD]]
[[Category: Gellman SH]]
[[Category: Haase HS]]
[[Category: Horne WS]]
[[Category: Kaufman KJ]]
[[Category: Lee EF]]
[[Category: Smith BJ]]