View source for 4a25


==X-ray structure Dps from Kineococcus radiotolerans in complex with Mn (II) ions.==
<StructureSection load='4a25' size='340' side='right'caption='[[4a25]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4a25]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Kineococcus_radiotolerans Kineococcus radiotolerans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A25 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4A25 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4a25 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a25 OCA], [https://pdbe.org/4a25 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4a25 RCSB], [https://www.ebi.ac.uk/pdbsum/4a25 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4a25 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A6WG04_KINRD A6WG04_KINRD] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
BACKGROUND: The ferroxidase center of DNA-binding protein from starved cells (Dps) is a major player in the iron oxidation/detoxification process that leads to a decreased reactive oxygen species production. The possible Mn(II) participation in this process has been studied in Dps from Kineococcus radiotolerans, a radiation-resistant bacterium with a high cytosolic Mn/Fe ratio and a high capacity to survive ionizing and stress conditions. METHODS: The X-ray structure of recombinant K. radiotolerans Dps loaded with Mn(II) has been solved at 2.0A resolution. Mn(II) binding to K. radiotolerans Dps and its effect on Fe(II) oxidation have been characterized in spectroscopic measurements. RESULTS: In K. radiotolerans Dps, the Fe-Fe ferroxidase center can have also a Mn-Fe composition. Mn(II) binds only at the high affinity, so-called A site, whereas Fe(II) binds also at the low affinity, so-called B site. The Mn-Fe and Fe-Fe centers behave distinctly upon iron oxidation by O(2). A site-bound Mn(II) or Fe(II) plays a catalytic role, while B site-bound Fe(II) behaves like a substrate and can be replaced by another Fe(II) after oxidation. When H(2)O(2) is the Fe(II) oxidant, single electrons are transferred to aromatic residues near the ferroxidase center and give rise to intra-protein radicals thereby limiting OH release in solution. The presence of the Mn-Fe center results in significant differences in the development of such intra-protein radicals. GENERAL SIGNIFICANCE: The results provide a likely molecular mechanism for the protective role of Mn(II) under oxidative stress conditions as it participates in redox cycling in the hetero-binuclear ferroxidase center.

Kineococcus radiotolerans Dps forms a heteronuclear Mn-Fe ferroxidase center that may explain the Mn-dependent protection against oxidative stress.,Ardini M, Fiorillo A, Fittipaldi M, Stefanini S, Gatteschi D, Ilari A, Chiancone E Biochim Biophys Acta. 2013 Feb 8. pii: S0304-4165(13)00046-9. doi:, 10.1016/j.bbagen.2013.02.003. PMID:23396000<ref>PMID:23396000</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4a25" style="background-color:#fffaf0;"></div>

==See Also==
*[[Ferritin 3D structures|Ferritin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Kineococcus radiotolerans]]
[[Category: Large Structures]]
[[Category: Ardini M]]
[[Category: Chiancone E]]
[[Category: Fiorillo A]]
[[Category: Ilari A]]
[[Category: Stefanini S]]