View source for 4aio


==Crystal structure of the starch debranching enzyme barley limit dextrinase==
<StructureSection load='4aio' size='340' side='right'caption='[[4aio]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4aio]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Hordeum_vulgare Hordeum vulgare]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AIO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AIO FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4aio FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4aio OCA], [https://pdbe.org/4aio PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4aio RCSB], [https://www.ebi.ac.uk/pdbsum/4aio PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4aio ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/O48541_HORVV O48541_HORVV] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Barley limit dextrinase (HvLD) is a debranching enzyme from glycoside hydrolase family 13 subfamily 13 (GH13_13) that hydrolyses alpha-1,6-glucosidic linkages in limit dextrins derived from amylopectin. The structure of HvLD was solved and refined to 1.9 A resolution. The structure has a glycerol molecule in the active site and is virtually identical to the structures of HvLD in complex with the competitive inhibitors alpha-cyclodextrin and beta-cyclodextrin solved to 2.5 and 2.1 A resolution, respectively. However, three loops in the N-terminal domain that are shown here to resemble carbohydrate-binding module family 21 were traceable and were included in the present HvLD structure but were too flexible to be traced and included in the structures of the two HvLD-inhibitor complexes.

Structure of the starch-debranching enzyme barley limit dextrinase reveals homology of the N-terminal domain to CBM21.,Moller MS, Abou Hachem M, Svensson B, Henriksen A Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Sep;68(Pt 9):1008-12. Epub, 2012 Aug 29. PMID:22949184<ref>PMID:22949184</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4aio" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Hordeum vulgare]]
[[Category: Large Structures]]
[[Category: Abou Hachem M]]
[[Category: Henriksen A]]
[[Category: Moeller MS]]
[[Category: Svensson B]]