View source for 4axo


==Structure of the Clostridium difficile EutQ protein==
<StructureSection load='4axo' size='340' side='right'caption='[[4axo]], [[Resolution|resolution]] 1.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4axo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridioides_difficile_630 Clostridioides difficile 630]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AXO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AXO FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4axo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4axo OCA], [https://pdbe.org/4axo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4axo RCSB], [https://www.ebi.ac.uk/pdbsum/4axo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4axo ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/EUTQ_CLOD6 EUTQ_CLOD6] A bidirectional acetate kinase that may drive flux through the ethanolamine (EA) degradation pathway under anoxic conditions found when this bacteria infects the host intestine. It may generate ATP that can be used by other enzymes (EutA and EutT) in the eut pathway. Might serve as an assembly hub for bacterial microcompartment (BMC) shell proteins (By similarity). Overexpression in E.coli leads to lamellar structures that inhibit cell division (PubMed:23144756). Expression of the eut operon may allow this bacteria to use EA as a carbon, nitrogen and energy source (Probable).[UniProtKB:Q9ZFV5]<ref>PMID:23144756</ref> <ref>PMID:23144756</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Bacterial microcompartments form a protective proteinaceous barrier around metabolic enzymes that process unstable or toxic chemical intermediates. The genome of the virulent, multidrug-resistant Clostridium difficile 630 strain contains an operon, eut, encoding a bacterial microcompartment with genes for the breakdown of ethanolamine and its utilisation as a source of reduced nitrogen and carbon. The C. difficile eut operon displays regulatory genetic elements and protein encoding regions in common with homologous loci found in the genomes of other bacteria, including the enteric pathogens Salmonella enterica and Enterococcus faecalis. The crystal structures of two microcompartment shell proteins, CD1908 and CD1918, and an uncharacterised protein with potential enzymatic activity, CD1925, were determined by X-ray crystallography. CD1908 and CD1918 display the same protein fold, though the order of secondary structure elements is permuted in CD1908 and this protein displays an N-terminal beta-strand extension. These proteins form hexamers with molecules related by crystallographic and non-crystallographic symmetry. The structure of CD1925 has a cupin beta-barrel fold and a putative active site that is distinct from the metal-ion dependent catalytic cupins. Thin-section transmission electron microscopy of Escherichia coli over-expressing eut proteins indicates that CD1918 is capable of self-association into arrays, suggesting an organisational role for CD1918 in the formation of this microcompartment. The work presented provides the basis for further study of the architecture and function of the C. difficile eut microcompartment, its role in metabolism and the wider consequences of intestinal colonisation and virulence in this pathogen.

Structural insight into the Clostridium difficile ethanolamine utilisation microcompartment.,Pitts AC, Tuck LR, Faulds-Pain A, Lewis RJ, Marles-Wright J PLoS One. 2012;7(10):e48360. doi: 10.1371/journal.pone.0048360. Epub 2012 Oct 29. PMID:23144756<ref>PMID:23144756</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 4axo" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Clostridioides difficile 630]]
[[Category: Large Structures]]
[[Category: Faulds-Pain A]]
[[Category: Lewis RJ]]
[[Category: Marles-Wright J]]
[[Category: Pitts AC]]
[[Category: Tuck LR]]