View source for 4axz


==Borrelia burgdorferi outer surface lipoprotein BBA73==
<StructureSection load='4axz' size='340' side='right'caption='[[4axz]], [[Resolution|resolution]] 2.09&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4axz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Borreliella_burgdorferi_B31 Borreliella burgdorferi B31]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AXZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AXZ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.09&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4axz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4axz OCA], [https://pdbe.org/4axz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4axz RCSB], [https://www.ebi.ac.uk/pdbsum/4axz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4axz ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/O50962_BORBU O50962_BORBU] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Borrelia burgdorferi, which is the causative agent of Lyme disease, is transmitted from infected Ixodes ticks to a mammalian host following a tick bite. Upon changing the host organism from an Ixodes tick to a warm-blooded mammal, the spirochete must adapt to very different conditions, which is achieved by altering the expression of several genes in response to a changing environment. Recently, considerable attention has been devoted to several outer surface proteins, including BBA73, that undergo dramatic upregulation during the transmission of B. burgdorferi from infected Ixodes ticks to mammals and that are thought to be important for the establishment and maintenance of the infection. These upregulated proteins could reveal the mechanism of pathogenesis and potentially serve as novel drug targets to prevent the transmission of the pathogenic bacteria. To promote effective treatments for Lyme disease and to gain better insight into B. burgdorferi pathogenesis, we have determined the crystal structure of the upregulated outer surface protein BBA73 at 2.0A resolution. We observed that the BBA73 protein exists as a homodimer both in the crystal and in solution. The monomers interact with their N-terminal alpha-helices and form a cleft that could potentially serve as a ligand or receptor binding site. To confirm that the protein dimerizes through the interaction of the N-terminal regions, we produced an N-terminal deletion mutant of BBA73 to disrupt dimerization, and we determined the crystal structure of the truncated BBA73 protein at 1.9A resolution. The truncated protein did not form a homodimer, and the crystal structure confirmed that the overall fold is identical to that of the native BBA73 protein. Notably, a paralogous protein CspA from B. burgdorferi with known crystal structure also forms a homodimer, albeit through an entirely different interaction between the monomers.

Structural characterization of the Borrelia burgdorferi outer surface protein BBA73 implicates dimerization as a functional mechanism.,Brangulis K, Petrovskis I, Kazaks A, Baumanis V, Tars K Biochem Biophys Res Commun. 2013 Apr 22. pii: S0006-291X(13)00654-2. doi:, 10.1016/j.bbrc.2013.04.028. PMID:23618869<ref>PMID:23618869</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4axz" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Borreliella burgdorferi B31]]
[[Category: Large Structures]]
[[Category: Baumanis V]]
[[Category: Brangulis K]]
[[Category: Kazaks A]]
[[Category: Petrovskis I]]
[[Category: Ranka R]]
[[Category: Tars K]]